Novel Blood Purification System for Regulating Excessive Immune Reactions in Severe Sepsis and Septic Shock: An Ex Vivo Pilot Study

Ther Apher Dial. 2015 Aug;19(4):308-15. doi: 10.1111/1744-9987.12338.


Promising results have been reported with blood purification as adjuvant treatment; however, the immunological mechanisms remain unclear. We have been developing a new blood purification system for regulating excessive immune reactions in severe sepsis and septic shock using a granulocyte adsorbing column (Adacolumn [Ada]), and a cytokine-adsorbing hemofilter (AN69ST hemofilter [AN69]). Fresh porcine blood was circulated for 6 h in five experimental groups including Ada and AN69 to assess the effects of leukocyte adsorption, phagocytic activity and adhesiveness of granulocytes. In the present study, we found that Ada mainly adsorbed granulocytes and monocytes, but not lymphocytes. The phagocytic activity level of granulocytes decreased, and adhesiveness increased, but the number of CD11b-positive cells markedly decreased in the current system. Elevated cytokine levels (IL-1β, IL-8 and IL-10) at the outlet of Ada were significantly lower than at the outlet of AN69 due to cytokine adsorption. Further studies are needed to better understand cellular interactions.

Keywords: Blood purification; Cytokine; Granulocyte; Immunomodulation; Sepsis.

MeSH terms

  • Adsorption
  • Animals
  • Disease Models, Animal
  • Hemodiafiltration* / instrumentation
  • Hemodiafiltration* / methods
  • Immunity, Cellular / physiology*
  • Interleukin-10 / blood
  • Interleukin-8 / blood
  • Leukocyte Count
  • Pilot Projects
  • Sepsis* / blood
  • Sepsis* / immunology
  • Sepsis* / therapy
  • Shock, Septic* / blood
  • Shock, Septic* / immunology
  • Shock, Septic* / therapy
  • Swine


  • IL10 protein, human
  • Interleukin-8
  • Interleukin-10