Spatiotemporal patterning of polyamines in Drosophila development

Amino Acids. 2015 Dec;47(12):2665-70. doi: 10.1007/s00726-015-2093-z. Epub 2015 Sep 19.

Abstract

While several studies have implicated polyamines (PAs) in development, little research has been done in genetically tractable model systems like Drosophila. Here, we integrate transcriptional and metabolic data across Drosophila development, and are the first to show temporal, stage-specific regulation of PA accumulation in embryonic trachea and eye discs using immunohistochemistry. Understanding the regulation driving this accumulation can provide insight into PA metabolism and transport. Our findings suggest that Drosophila has great potential for investigating PAs in developmental biology.

Keywords: Drosophila; Embryogenesis; Eye imaginal disc; Polyamine metabolism; Spermidine; Spermine; Trachea.

MeSH terms

  • Animals
  • Body Patterning
  • Computational Biology
  • Drosophila melanogaster / embryology*
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Green Fluorescent Proteins / metabolism
  • Immunohistochemistry
  • Photoreceptor Cells, Invertebrate / physiology
  • Polyamines / chemistry*
  • Spermidine / chemistry
  • Spermine / chemistry
  • Time Factors
  • Trachea / embryology
  • Transcription, Genetic

Substances

  • Polyamines
  • Green Fluorescent Proteins
  • Spermine
  • Spermidine