Selective Binding of DNA Origami on Biomimetic Lipid Patches

Michael Hirtz; Josipa Brglez; Harald Fuchs; Christof M Niemeyer

doi:10.1002/smll.201501333

Selective Binding of DNA Origami on Biomimetic Lipid Patches

Small. 2015 Nov 18;11(43):5752-8. doi: 10.1002/smll.201501333. Epub 2015 Sep 21.

Authors

Michael Hirtz¹, Josipa Brglez², Harald Fuchs^{1

3}, Christof M Niemeyer²

Affiliations

¹ Institute of Nanotechnology (INT) and Karlsruhe Nano Micro Facility (KNMF), Karlsruhe Institute of Technology (KIT), 76128, Karlsruhe, Germany.
² Institute for Biological Interfaces (IBG 1), Karlsruhe Institute of Technology (KIT), 76128, Karlsruhe, Germany.
³ Physical Institute and Center for Nanotechnology (CeNTech), Westfälische Wilhelms-Universität, 48149, Münster, Germany.

PMID: 26389563
DOI: 10.1002/smll.201501333

Abstract

Arrays of biomimetic lipid patches for studying the binding of DNA origami structures can be tailored in size, shape, and composition with the aid of lipid-dip pen nanolithography. This approach allows for analysis of the effects of lipid composition with high throughput which could be applied for the targeted presentation of functional DNA origami structures on surfaces.

Keywords: DNA origami; biomimetic membranes; dip-pen nanolithography; phospholipids; supported lipid bilayer membranes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adhesiveness
Binding Sites
Biomimetic Materials / chemistry*
DNA / chemistry*
DNA / ultrastructure*
Lipid Bilayers / chemistry*
Materials Testing / methods
Microarray Analysis / methods*
Molecular Imprinting / methods*
Nucleic Acid Conformation
Phosphatidylcholines / chemistry
Structure-Activity Relationship

Substances

Lipid Bilayers
Phosphatidylcholines
DNA
1,2-oleoylphosphatidylcholine