Anti-Oxidant, Anti-Aging, and Anti-Melanogenic Properties of the Essential Oils from Two Varieties of Alpinia zerumbet

Molecules. 2015 Sep 14;20(9):16723-40. doi: 10.3390/molecules200916723.


Here, we investigated the anti-oxidant and anti-aging effects of essential oils (EOs) from the leaves of Alpinia zerumbet (tairin and shima) in vitro and anti-melanogenic effects in B16F10 melanoma cells. The anti-oxidant activities were performed with 2,2-diphenyl-1-picrylhydrazyl (DPPH); 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS); nitric oxide; singlet oxygen; hydroxyl radical scavenging; and xanthine oxidase. The inhibitory activities against collagenase, elastase, hyaluronidase, and tyrosinase were employed for anti-aging. The anti-melanogenic was assessed in B16F10 melanoma cells by melanin synthesis and intracellular tyrosinase inhibitory activity. The volatile chemical composition of the essential oil was analyzed with gas chromatography-mass spectrometry (GC/MS). The EO was a complex mixture mainly consisting of monoterpenes and sesquiterpenes. The results revealed that tairin and shima EOs showed strong anti-oxidant activities against DPPH and nitric oxide, hydroxyl radical scavenging activity, and xanthine oxidase inhibition. Compared to shima EO; tairin EO exhibited strong anti-aging activity by inhibiting collagenase, tyrosinase, hyaluronidase, and elastase (IC50 = 11 ± 0.1; 25 ± 1.2; 83 ± 1.6; and 213 ± 2 μg/mL, respectively). Both EOs inhibited intracellular tyrosinase activity; thus, reducing melanin synthesis. These results suggest that tairin EO has better anti-oxidant/anti-aging activity than shima EO, but both are equally anti-melanogenic.

Keywords: Alpinia zerumbet; anti-aging; anti-melanogenic; anti-oxidant; essential oil.

MeSH terms

  • Aging / drug effects*
  • Alpinia / chemistry*
  • Alpinia / classification
  • Antioxidants / chemistry
  • Antioxidants / pharmacology*
  • Cell Survival / drug effects
  • Free Radical Scavengers / chemistry
  • Free Radical Scavengers / pharmacology
  • Gas Chromatography-Mass Spectrometry
  • Humans
  • Melanins / chemistry
  • Melanins / pharmacology*
  • Melanoma, Experimental / drug therapy*
  • Monophenol Monooxygenase / antagonists & inhibitors*
  • Oils, Volatile / pharmacology*
  • Tumor Cells, Cultured


  • Antioxidants
  • Free Radical Scavengers
  • Melanins
  • Oils, Volatile
  • Monophenol Monooxygenase