Fabrication of Robust Protein Films Using Nanoimprint Lithography

Eunhee Jeoung; Bradley Duncan; Li-Sheng Wang; Krishnendu Saha; Chandramouleeswaran Subramani; Peijian Wang; Yi-Cheun Yeh; Takashi Kushida; Yoni Engel; Michael D Barnes; Vincent M Rotello

doi:10.1002/adma.201502457

Fabrication of Robust Protein Films Using Nanoimprint Lithography

Adv Mater. 2015 Oct 28;27(40):6251-5. doi: 10.1002/adma.201502457. Epub 2015 Sep 21.

Affiliations

¹ Department of Chemistry, Gangneung-Wonju National University, Gangneung, Gangwon-do, 210-702, South Korea.
² Department of Chemistry, University of Massachusetts Amherst, Amherst, MA, 01003, USA.
³ Department of Physics, University of Massachusetts Amherst, Amherst, MA, 01003, USA.
⁴ Integrative Technology Research Institute, Teijin Limited, 4-3-2 Asahigaoka, Hino, Tokyo, 191-8512, Japan.

PMID: 26390179
DOI: 10.1002/adma.201502457

Abstract

A nanoimprint-lithography-based fabrication method to generate stable protein films is described. The process is environmentally friendly and generalizable with respect to the protein building blocks. These non-fouling surfaces are readily patternable, incorporate intrinsic protein charge into the film, and able to control cellular adhesion.

Keywords: antifouling; controlled cellular adhesion; nanoimprint lithography; protein films; surface modification.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

3T3 Cells
Animals
Cattle
Cell Adhesion
Circular Dichroism
Fibroblasts / cytology
Fibroblasts / physiology
Hemoglobins / chemistry
Mice
Microscopy, Atomic Force
Microscopy, Fluorescence
Muramidase / chemistry
Nanostructures*
Pressure
Proteins* / chemical synthesis
Proteins* / chemistry
Serum Albumin, Bovine / chemistry
Surface Properties
Temperature
Water / chemistry

Substances

Hemoglobins
Proteins
Water
Serum Albumin, Bovine
Muramidase