Genomic and chromosomal damage in the marine mussel Mytilus galloprovincialis: Effects of the combined exposure to titanium dioxide nanoparticles and cadmium chloride

L Rocco; M Santonastaso; M Nigro; F Mottola; D Costagliola; M Bernardeschi; P Guidi; P Lucchesi; V Scarcelli; I Corsi; V Stingo; G Frenzilli

doi:10.1016/j.marenvres.2015.09.004

Genomic and chromosomal damage in the marine mussel Mytilus galloprovincialis: Effects of the combined exposure to titanium dioxide nanoparticles and cadmium chloride

Mar Environ Res. 2015 Oct:111:144-8. doi: 10.1016/j.marenvres.2015.09.004. Epub 2015 Sep 5.

Authors

L Rocco¹, M Santonastaso², M Nigro³, F Mottola², D Costagliola², M Bernardeschi³, P Guidi³, P Lucchesi³, V Scarcelli³, I Corsi⁴, V Stingo², G Frenzilli³

Affiliations

¹ Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, Caserta, Italy. Electronic address: lucia.rocco@unina2.it.
² Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, Caserta, Italy.
³ Department of Clinical and Experimental Medicine, Pisa University, Pisa, Italy.
⁴ Department of Physical, Earth and Environmental Sciences, University of Siena, Siena, Italy.

PMID: 26392349
DOI: 10.1016/j.marenvres.2015.09.004

Abstract

Titanium dioxide nanoparticles (TiO2-NPs) continuously released into waters, may cause harmful effects to marine organisms and their potential interaction with conventional toxic contaminants represents a growing concern for biota. We investigated the genotoxic potential of nanosized titanium dioxide (n-TiO2) (100 μg L(-1)) alone and in combination with CdCl2 (100 μg L(-1)) in Mytilus galloprovincialis after 4 days of in vivo exposure. RAPD-PCR technique and Micronucleus test were used to study genotoxicity. The results showed genome template stability (GTS) being markedly reduced after single exposure to n-TiO2 and CdCl2. Otherwise, co-exposure resulted in a milder reduction of GTS. Exposure to n-TiO2 was responsible for a significant increase of micronucleated cell frequency in gill tissue, while no chromosomal damage was observed after CdCl2 exposure as well as after combined exposure to both substances.

Keywords: Cadmium; DNA adducts; Genotoxicity; Metal interactions; Mytilus galloprovincialis; NanoTiO(2); Pollution effects.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cadmium Chloride / toxicity*
Metal Nanoparticles / toxicity*
Micronucleus Tests
Mutagens / toxicity*
Mytilus / drug effects*
Random Amplified Polymorphic DNA Technique
Titanium / toxicity*

Substances

Mutagens
titanium dioxide
Titanium
Cadmium Chloride