Effect of blood vessels on light distribution in optogenetic stimulation of cortex

Opt Lett. 2015 May 15;40(10):2173-6. doi: 10.1364/OL.40.002173.


In this Letter, the impact of blood vessels on light distribution during photostimulation of cortical tissue in small rodents is investigated. Brain optical properties were extracted using a double-integrating sphere setup, and optical coherence tomography was used to image cortical vessels and capillaries to generate a three-dimensional angiogram of the cortex. By combining these two datasets, a complete volumetric structure of the cortical tissue was developed and linked to a Monte Carlo code which simulates light propagation in this inhomogeneous structure and illustrates the effect of blood vessels on the penetration depth and pattern preservation in optogenetic stimulation.

MeSH terms

  • Animals
  • Blood Vessels / radiation effects*
  • Brain / blood supply*
  • Brain / metabolism
  • Brain / radiation effects
  • Light*
  • Monte Carlo Method
  • Optogenetics*
  • Rats
  • Tomography, Optical Coherence