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, 30 (6), 880-7

Variations in Chemical Fingerprints and Major Flavonoid Contents From the Leaves of Thirty-One Accessions of Hibiscus Sabdariffa L

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Variations in Chemical Fingerprints and Major Flavonoid Contents From the Leaves of Thirty-One Accessions of Hibiscus Sabdariffa L

Jin Wang et al. Biomed Chromatogr.

Abstract

The leaves of Hibiscus sabdariffa L. have been used as traditional folk medicines for treating high blood pressure and fever. There are many accessions of H. sabdariffa L. throughout the world. To assess the chemical variations of 31 different accessions of H. sabdariffa L., fingerprinting analysis and quantitation of major flavonoids were performed by high-performance liquid chromatography (HPLC). The HPLC method was validated for linearity, sensitivity, precision, repeatability and accuracy. A quadrupole-time-of-flight mass spectrometry (Q-TOF-MS) was applied for the characterization of major compounds. A total of 9 compounds were identified, including 6 flavonoids and 3 phenolic acids. In the fingerprint analysis, similarity analysis (SA) and principal component analysis (PCA) were used to differentiate the 31 accessions of H. sabdariffa L. Based on the results of PCA and SA, the samples No. 15 and 19 appeared much different from the main group. The total content of five flavonoids varied greatly among different accessions, ranging from 3.35 to 23.30 mg/g. Rutin was found to be the dominant compound and the content of rutin could contribute to chemical variations among different accessions. This study was helpful to understand the chemical variations between different accessions of H. sabdariffa L., which could be used for quality control. © 2015 The Authors Biomedical Chromatography Published by John Wiley & Sons Ltd.

Keywords: Hibiscus sabdariffa L; LC-Q-TOF-MS; fingerprints; flavonoids; principal components analysis (PCA).

Figures

Figure 1
Figure 1
Structures of five investigated flavonoids.
Figure 2
Figure 2
Chromatograms of mixed standards (A) including: rutin (4), kaempferol‐3‐o‐rutinoside (6), kaempferol‐3‐o‐glucoside (7), quercetin (8), kaempferol (9), and (B) common pattern of HPLC fingerprint of 31 samples of H. sabdariffa L.
Figure 3
Figure 3
The HPLC fingerprints of the 31 H. sabdariffa L. samples at wavelength 350 nm.
Figure 4
Figure 4
PC1‐PC2 score plot of 31 samples. The labels of the samples refer to Table 1.
Figure 5
Figure 5
Similarity evaluation of HPLC fingerprint for H. sabdariffa L. Different samples (No.1‐31) are listed in Table 1.

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