A short report: PAMM, a novel antioxidant protein, induced by oxidative stress

Redox Biol. 2015 Dec:6:446-453. doi: 10.1016/j.redox.2015.09.008. Epub 2015 Sep 23.

Abstract

Reactive oxygen species (ROS) play a central role in estrogen deficiency-induced bone loss. We previously identified and characterized a novel member of the Peroxiredoxin (PRX) like 2 family that we called PAMM: Peroxiredoxin Activated in M-CSF stimulated Monocytes, a redox regulatory protein that modulates osteoclast differentiation in vitro. In this study, we report increased PAMM expression in H2O2-treated cells and in bones from ovariectomized (OVX) mice 4 weeks after surgery, models for oxidative stress in vitro and in vivo, respectively. We also detected increased PAMM abundance and phosphorylated Akt in OVX mice treated with estrogen. In addition, Wortmannin, a specific PI3Kinase inhibitor and Rapamycin, an inhibitor of the PI3Kinase/Akt pathway, blocked Akt phosphorylation and stimulation of PAMM expression by M-CSF. These results indicate that M-CSF-induced PAMM expression is mediated by Akt phosphorylation. Our data also suggest that estrogen-induced PAMM expression is mediated by phosphorylation of Akt. These findings point to PAMM as a potential candidate for Akt-mediated protection against oxidative stress.

Keywords: Antioxidant; Bone loss; Ovariectomy; Oxidative stress; PAMM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / metabolism
  • Bone Density
  • Cells, Cultured
  • Estrogens / physiology
  • Female
  • Humans
  • Macrophage Colony-Stimulating Factor / physiology
  • Mice
  • NF-E2-Related Factor 2 / metabolism
  • Osteoclasts / metabolism
  • Ovariectomy
  • Oxidation-Reduction
  • Oxidative Stress*
  • Peroxiredoxins / genetics
  • Peroxiredoxins / metabolism*
  • Phosphorylation
  • Protein Processing, Post-Translational
  • Proto-Oncogene Proteins c-akt / metabolism
  • Transcriptional Activation

Substances

  • Antioxidants
  • Estrogens
  • NF-E2-Related Factor 2
  • NFE2L2 protein, human
  • PRXL2A protein, human
  • Macrophage Colony-Stimulating Factor
  • Peroxiredoxins
  • Proto-Oncogene Proteins c-akt