T-cell receptor profiling in cancer

Mol Oncol. 2015 Dec;9(10):2063-70. doi: 10.1016/j.molonc.2015.09.003. Epub 2015 Sep 15.


Immunosequencing is a platform technology that allows the enumeration, specification and quantification of each and every B- and/or T-cell in any biologic sample of interest. Thus, it provides an assessment of the level and distribution of all the clonal lymphocytes in any sample, and allows "tracking" of a single clone or multiple clones of interest over time or from tissue to tissue within a given patient. It is based on bias-controlled multiplex PCR and high-throughput sequencing, and it is highly accurate, standardized, and sensitive. In this review, we provide evidence that immunosequencing is becoming an important analytic tool for the emerging field of immune-oncology, and describe several applications of this approach, including the assessment of residual disease post therapy in lymphoid malignancies, the prediction of response to immunotherapeutics of solid tumors containing tumor infiltrating lymphocytes, the identification of clonal responses in vaccination, infectious disease, bone marrow reconstitution, and autoimmunity, and the exploration of whether there are population-based stereotyped responses to certain exposures or interventions.

Keywords: Ig/TCR sequence biomarkers; Immunosequencing; Lymphoid clonality assessment/tracking.

Publication types

  • Review

MeSH terms

  • Humans
  • Neoplasms / immunology*
  • Receptors, Antigen, T-Cell / immunology*


  • Receptors, Antigen, T-Cell