Associations of miR-146a and miR-146b expression and breast cancer in very young women

Cancer Biomark. 2015;15(6):881-7. doi: 10.3233/CBM-150532.

Abstract

Background: MicroRNAs (miRNA) expression profiles might be useful novel biomarkers for tumor diagnostic and histological characterization.

Objective: Associations of miR-146 expression and breast cancer in very young women needed to be elucidated.

Methods: We investigated expressions of miR-146a, miR-146b and IL-6 in tissues between 120 young women with primary breast cancer and 130 patients with breast fibroadenoma by RT-PCR. The associations between the expression of miR-146, IL-6 and clinical parameters of breast cancer were analyzed.

Results: Levels of miR-146a and miR-146b in breast cancer tissues were lower while level of IL-6 was higher compared to the fibroadenoma tissues and pericancerous breast tissues (P< 0.05). Positive associations were found between levels of miR-146a/b and IL-6 in breast cancer tissues and ER-PgR-, HER2/neu-, Ki-67 index ≥ 20%, tumor size > 2 cm, positive distant metastasis and lymph node metastasis, advanced clinical TNM stages (III-IV) and basal-like phenotype (P< 0.05).

Conclusion: Down-regulations of miR-146a and miR-146b expression in breast tissues were related to development and deterioration of breast cancer. miR-146a and miR-146b might act as potential biomarkers for young women with breast cancer.

Keywords: Breast cancer; RT-PCR; miR-146a; miR-146b.

MeSH terms

  • Adult
  • Biomarkers, Tumor / genetics*
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / pathology
  • Female
  • Follow-Up Studies
  • Humans
  • Interleukin-6 / genetics*
  • Lymphatic Metastasis
  • MicroRNAs / genetics*
  • Neoplasm Invasiveness
  • Neoplasm Staging
  • Prognosis

Substances

  • Biomarkers, Tumor
  • Interleukin-6
  • MIRN146 microRNA, human
  • MicroRNAs