The oil-resin of the tropical rainforest tree Copaifera langsdorffii reduces cell viability, changes cell morphology and induces cell death in human endometriotic stromal cultures

J Pharm Pharmacol. 2015 Dec;67(12):1744-55. doi: 10.1111/jphp.12479. Epub 2015 Sep 26.

Abstract

Objectives: The hormonal treatment for endometriosis frequently fails to completely eradicate endometriotic implants. A new therapeutic treatment is needed. This study investigates the in-vitro effect of Copaifera langsdorffii oil-resin on human eutopic and ectopic endometrium stromal cell cultures (EuESCs and EctESCs).

Methods: A nanocomposite system containing the copaiba oil-resin (NanoCOR) was developed and acute toxicity test was performed. Endometrial stromal cells (ESCs) from non-endometriotics controls (CESCs), EuESCs and EctESCs were isolated and treated with different concentrations of NanoCOR, at different time intervals to evaluate its effect on cell morphology, proliferation, viability, necrosis and apoptosis induction.

Key findings: When treated with 50 μg/ml of NanoCOR, the morphology of EctESCs changed, as the actin microfilaments were disorganized, disassembled or disrupted. Moreover, at 24 h of treatment with NanoCOR, the EctESCs viability was inhibited, and a significant number of these cells underwent apoptosis. In EuESCs, these effects were observed only at 48 h. Finally, the treatment of EctESCs with NanoCOR increased the lactate dehydrogenase release into the extracellular medium more than in EuESCs.

Conclusions: Our data indicate that NanoCOR has a greater impact on the behaviour of human endometriotic stromal cells than on the eutopic endometrium stromal cells, supporting the idea that NanoCOR should be further investigated as a novel and valuable alternative to treat endometriosis.

Keywords: copaiba oil-resin; ectopic endometrial stromal cells; endometriosis; eutopic endometrial stromal cells; nanocomposite.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actin Cytoskeleton / drug effects
  • Actin Cytoskeleton / pathology
  • Animals
  • Apoptosis / drug effects
  • Case-Control Studies
  • Cell Proliferation / drug effects
  • Cell Shape / drug effects*
  • Cell Survival / drug effects
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Endometriosis / drug therapy*
  • Endometriosis / pathology
  • Endometrium / drug effects*
  • Endometrium / pathology
  • Fabaceae / chemistry*
  • Female
  • Humans
  • Male
  • Mice
  • Nanoparticles
  • Necrosis
  • Phytotherapy
  • Plant Oils / isolation & purification
  • Plant Oils / pharmacology*
  • Plant Oils / toxicity
  • Plants, Medicinal
  • Rainforest
  • Resins, Plant / isolation & purification
  • Resins, Plant / pharmacology*
  • Resins, Plant / toxicity
  • Stromal Cells / drug effects*
  • Stromal Cells / pathology
  • Time Factors
  • Trees*
  • Tropical Climate

Substances

  • Plant Oils
  • Resins, Plant