Vitamin D metabolite profiling using liquid chromatography-tandem mass spectrometry (LC-MS/MS)

J Steroid Biochem Mol Biol. 2016 Nov:164:110-114. doi: 10.1016/j.jsbmb.2015.09.026. Epub 2015 Sep 26.

Abstract

Liquid chromatography tandem mass spectrometry (LC-MS/MS) has emerged as the latest technology to be used to assay the metabolites of vitamin D. The method uses molecular mass as a detection technique after straightforward extraction and chromatography steps. LC-MS/MS assay provides a level of accuracy and reproducibility not seen before with other methods and is beginning to rival antibody-based methods in terms of sensitivity and convenience. Methods for detection of underivatized and DMEQ-TAD derivatized vitamin D metabolites are evaluated. Sensitivity is improved by 10-100 fold with derivatization and allows for the simultaneous assay of multiple vitamin D metabolites, a process termed vitamin D metabolite profiling. Clinical and research applications of vitamin D metabolite profiling are discussed.

Keywords: 25-OH-D(3); DMEQ-TAD; LC–MS/MS; Metabolomics; Vitamin D assay.

Publication types

  • Review
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding, Competitive
  • Calcifediol / metabolism
  • Chromatography, Liquid*
  • Female
  • Genotype
  • Guinea Pigs
  • Humans
  • Male
  • Mice
  • Mice, Knockout
  • Protein Binding
  • Quinoxalines / chemistry
  • Radioimmunoassay
  • Reproducibility of Results
  • Tandem Mass Spectrometry*
  • Triazoles / chemistry
  • Vitamin D / metabolism*

Substances

  • Quinoxalines
  • Triazoles
  • 4-(2-(6,7-dimethoxy-4-methyl-3-oxo-3,4-dihydroquinoxalinyl)ethyl)-1,2,4-triazoline-3,5-dione
  • Vitamin D
  • Calcifediol

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