Presenting native-like HIV-1 envelope trimers on ferritin nanoparticles improves their immunogenicity

Retrovirology. 2015 Sep 26;12:82. doi: 10.1186/s12977-015-0210-4.

Abstract

Background: Presenting vaccine antigens in particulate form can improve their immunogenicity by enhancing B cell activation.

Findings: We describe ferritin-based protein nanoparticles that display multiple copies of native-like HIV-1 envelope glycoprotein trimers (BG505 SOSIP.664). Trimer-bearing nanoparticles were significantly more immunogenic than trimers in both mice and rabbits. Furthermore, rabbits immunized with the trimer-bearing nanoparticles induced significantly higher neutralizing antibody responses against most tier 1A viruses, and higher responses (but not significantly), to several tier 1B viruses and the autologous tier 2 virus than when the same trimers were delivered as soluble proteins.

Conclusions: This or other nanoparticle designs may be practical ways to improve the immunogenicity of envelope glycoprotein trimers.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • AIDS Vaccines / chemistry
  • AIDS Vaccines / immunology
  • Animals
  • Antibodies, Neutralizing / biosynthesis*
  • Female
  • Ferritins / immunology
  • HIV Antibodies / biosynthesis*
  • HIV-1 / chemistry*
  • HIV-1 / immunology*
  • Humans
  • Immunization
  • Mice
  • Nanoparticles
  • Protein Multimerization
  • Rabbits
  • env Gene Products, Human Immunodeficiency Virus / chemistry
  • env Gene Products, Human Immunodeficiency Virus / immunology*

Substances

  • AIDS Vaccines
  • Antibodies, Neutralizing
  • HIV Antibodies
  • env Gene Products, Human Immunodeficiency Virus
  • Ferritins