Piperine Ameliorates Lipopolysaccharide-Induced Acute Lung Injury via Modulating NF-κB Signaling Pathways

Inflammation. 2016 Feb;39(1):303-308. doi: 10.1007/s10753-015-0250-x.


Piperine, one of the active components of black pepper, has been reported to have antioxidant and anti-inflammatory activities. However, the effects of piperine on lipolysaccharide (LPS)-induced acute lung injury (ALI) have not been reported. Thus, the protective effects of piperine against LPS-induced ALI were investigated in this study. LPS-induced lung injury was assessed by histological study, myeloperoxidase (MPO) activity, and inflammatory cytokine production. Our results demonstrated that piperine attenuated LPS-induced MPO activity, lung edema, and inflammatory cytokines TNF-α, IL-6, and IL-1β production. Histological studies showed that piperine obviously attenuated LPS-induced lung injury. In addition, piperine significantly inhibited LPS-induced NF-κB activation. In conclusion, our results demonstrated that piperine had a protective effect on LPS-induced ALI. The anti-inflammatory mechanism of piperine is through inhibition of NF-κB activation. Piperine may be a potential therapeutic agent for ALI.

Keywords: LPS; NF-κB; acute lung injury; piperine.

MeSH terms

  • Acute Lung Injury / drug therapy*
  • Alkaloids / pharmacology*
  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Antioxidants / pharmacology*
  • Benzodioxoles / pharmacology*
  • Enzyme Activation / drug effects
  • Interleukin-1beta / biosynthesis
  • Interleukin-6 / biosynthesis
  • Lipopolysaccharides
  • Lung Injury / chemically induced
  • Lung Injury / drug therapy*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • NF-kappa B / metabolism*
  • Neutrophil Infiltration / drug effects
  • Peroxidase / metabolism
  • Piperidines / pharmacology*
  • Polyunsaturated Alkamides / pharmacology*
  • Pulmonary Edema / drug therapy*
  • Signal Transduction / drug effects
  • Tumor Necrosis Factor-alpha / biosynthesis


  • Alkaloids
  • Anti-Inflammatory Agents
  • Antioxidants
  • Benzodioxoles
  • IL1B protein, mouse
  • Interleukin-1beta
  • Interleukin-6
  • Lipopolysaccharides
  • NF-kappa B
  • Piperidines
  • Polyunsaturated Alkamides
  • Tumor Necrosis Factor-alpha
  • Peroxidase
  • piperine