Fitness Studies of Azole-Resistant Strains of Aspergillus fumigatus

Antimicrob Agents Chemother. 2015 Dec;59(12):7866-9. doi: 10.1128/AAC.01594-15. Epub 2015 Sep 28.

Abstract

Isogenic bar-coded strains of Aspergillus fumigatus carrying the G54W or M220K mutation in Cyp51A were constructed. In vitro, the growth and conidiation capacities of the mutants were similar to those of the parental strain. Competition studies in the absence of azoles showed that there was no adverse fitness cost for the azole-resistant A. fumigatus strains in vitro or in vivo compared to the parental strain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antifungal Agents / pharmacology*
  • Aspergillosis / drug therapy
  • Aspergillosis / microbiology
  • Aspergillosis / pathology
  • Aspergillus fumigatus / drug effects*
  • Aspergillus fumigatus / genetics*
  • Aspergillus fumigatus / growth & development
  • Aspergillus fumigatus / pathogenicity
  • Azoles / pharmacology*
  • Cytochrome P-450 Enzyme System / genetics*
  • Cytochrome P-450 Enzyme System / metabolism
  • Drug Resistance, Fungal / genetics*
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Gene Expression
  • Genetic Fitness
  • Lung / drug effects
  • Lung / microbiology
  • Male
  • Mice
  • Mutation
  • Spores, Fungal / drug effects
  • Spores, Fungal / enzymology
  • Spores, Fungal / growth & development
  • Spores, Fungal / pathogenicity
  • Virulence

Substances

  • Antifungal Agents
  • Azoles
  • Fungal Proteins
  • Cytochrome P-450 Enzyme System
  • cytochrome P-450 CYP51A, Aspergillus