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. 2015 Sep 30;4:e07574.
doi: 10.7554/eLife.07574.

Mapping the Conformational Landscape of a Dynamic Enzyme by Multitemperature and XFEL Crystallography

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Free PMC article

Mapping the Conformational Landscape of a Dynamic Enzyme by Multitemperature and XFEL Crystallography

Daniel A Keedy et al. Elife. .
Free PMC article

Abstract

Determining the interconverting conformations of dynamic proteins in atomic detail is a major challenge for structural biology. Conformational heterogeneity in the active site of the dynamic enzyme cyclophilin A (CypA) has been previously linked to its catalytic function, but the extent to which the different conformations of these residues are correlated is unclear. Here we compare the conformational ensembles of CypA by multitemperature synchrotron crystallography and fixed-target X-ray free-electron laser (XFEL) crystallography. The diffraction-before-destruction nature of XFEL experiments provides a radiation-damage-free view of the functionally important alternative conformations of CypA, confirming earlier synchrotron-based results. We monitored the temperature dependences of these alternative conformations with eight synchrotron datasets spanning 100-310 K. Multiconformer models show that many alternative conformations in CypA are populated only at 240 K and above, yet others remain populated or become populated at 180 K and below. These results point to a complex evolution of conformational heterogeneity between 180--240 K that involves both thermal deactivation and solvent-driven arrest of protein motions in the crystal. The lack of a single shared conformational response to temperature within the dynamic active-site network provides evidence for a conformation shuffling model, in which exchange between rotamer states of a large aromatic ring in the middle of the network shifts the conformational ensemble for the other residues in the network. Together, our multitemperature analyses and XFEL data motivate a new generation of temperature- and time-resolved experiments to structurally characterize the dynamic underpinnings of protein function.

Keywords: biophysics; enzymology; human; protein dynamics; structural biology; x-ray free electron laser.

Conflict of interest statement

ATB: Reviewing editor, eLife. The other authors declare that no competing interests exist.

Figures

Figure 1.
Figure 1.. Automated electron density sampling reveals increased conformational redistribution.
Ringer curves of 2mFo-DFc electron density versus χ1 dihedral angle for (A) Ser99 and (B) Leu98 show large peaks for modeled major conformations and smaller peaks for additional minor conformations (dashed vertical lines). These secondary peaks become more evident as temperature increases (color gradient from blue to purple to red). A backrub motion was used for Ser99. For (C) Ser99 and (D) Leu98, a Pearson correlation coefficient was calculated between each pair of Ringer curves from the corresponding panel in (A) or (B). Circles in diagonal elements are colored as in (A) or (B); circles in off-diagonal elements are all gray but scaled by pairwise correlation coefficient (see legend). Pairs of curves from similar temperatures are generally more correlated to each other (larger circles) than are pairs of curves from more different temperatures (smaller circles). DOI: http://dx.doi.org/10.7554/eLife.07574.004
Figure 1—Figure supplement 1.
Figure 1—Figure supplement 1.. Radiation damage is minimal across data collection temperatures.
Plots of Rd versus frame-number difference for each dataset in the multitemperature trajectory reveal only minimal radiation damage. The datasets around 180– 260 K exhibit higher Rd in later frames, which may reflect either a time-dependent cryocooling artifact or a radiation damage at these intermediate temperatures. Although the rate of X-ray damage varies strongly with temperature, the data collection strategy was adjusted to yield a comparable amount of damage per frame. Therefore, there is no correlation between data collection temperature and the overall extent of radiation damage; the highest temperature datasets are equally undamaged as the lowest temperature datasets. By contrast, we observe a strong correlation between data collection temperature and conformational heterogeneity. DOI: http://dx.doi.org/10.7554/eLife.07574.005
Figure 2.
Figure 2.. Multiconformer modeling across temperatures captures increasing conformational heterogeneity.
Residues extending from the core to the active site of cyclophilin A (CypA) adopt a single conformer at low temperatures, but gradually transition to increasing occupancy of secondary conformations as temperature increases. These conformations are well supported by 2mFo-DFc electron density contoured at 0.6 σ (cyan mesh) and 3.0 σ (dark blue mesh). This is corroborated by the room-temperature X-ray free-electron laser (XFEL) model (gray), which is free from conventional radiation damage and features the same secondary conformations. Water molecules (red spheres) are more fully ordered at low temperatures, but become only partially occupied at higher temperatures because they are mutually exclusive with the secondary Phe113 conformation. DOI: http://dx.doi.org/10.7554/eLife.07574.008
Figure 2—figure supplement 1.
Figure 2—figure supplement 1.. Single-conformer models cannot explain the crystallographic data at higher temperatures..
The CypA dynamic network is shown after molecular replacement and refinement (including automated water placement) in PHENIX, before any manual rebuilding. The major state is well supported by 2mFo-DFc electron density contoured at 0.6σ (cyan mesh) and 3.0σ (dark blue mesh) for all datasets, but mFo-DFc difference electron density becomes more negative for the major state (−3.0σ, red mesh) and more positive for the unmodeled minor state ( 3.0σ, green mesh) as temperature increases across the synchrotron datasets (blue to red), especially at and above 240 K. Full-occupancy water molecules (red spheres) are automatically placed by PHENIX near the Phe113 minor state in lower temperature, but not in higher temperature synchrotron models because they are mutually exclusive with the secondary Phe113 conformation. DOI: http://dx.doi.org/10.7554/eLife.07574.009
Figure 3.
Figure 3.. The active-site conformational ensemble of cyclophilin A (CypA) determined without radiation damage at room temperature.
(A) Electron density maps for room-temperature synchrotron (red) and (B) X-ray-free electron laser (XFEL) (silver) single-conformer models reveal conformational heterogeneity extending from the protein core (Leu98 and Ser99) to the active site (Arg55) of CypA. The primary conformation is well supported by 2mFo-DFc electron density contoured at 0.6 σ (cyan mesh) and 3.0 σ (dark blue mesh). mFo-DFc difference electron density contoured at 3.0 σ (green mesh) and − 3.0 σ (red mesh) suggests unmodeled alternative conformations. (C, D) Finalized multiconformer models explicitly model these alternative conformations, which are well-supported by 2mFo-DFc electron density. (E, F) Ringer electron density sampling for the single-conformer models shows peaks representing alternative conformations for (E) Ser99 and (F) Leu98. The primary conformations of both residues are obvious as peaks for both models, but the minor conformations (dashed vertical line; as modeled in 3k0n) are also evident, with 2mFo-DFc values well above the 0.3σ (darker gray horizontal line) threshold, except for the Leu98 in the XFEL model (due to the lower resolution). A backrub motion of −10° positions the backbone properly for Ringer to best detect the minor conformation for Ser99, but not for Leu98. DOI: http://dx.doi.org/10.7554/eLife.07574.013
Figure 3—figure supplement 1.
Figure 3—figure supplement 1.. The 1.2 Å room-temperature CypA synchrotron data show no signs of radiation damage.
A plot of ‘decay R-factor’ (Rd) as a function of frame-number difference (as in Figure 1—figure supplement 2) has a slope of zero, indicating the absence of radiation damage. Rd is calculated using pairwise observations of unique reflections (hkl) with centroids on frames i and j, and the frame-number difference is given by i-j. The calculations were performed using a 2.0 Å resolution cutoff. DOI: http://dx.doi.org/10.7554/eLife.07574.014
Figure 4.
Figure 4.. Alternative loop conformations can appear at lower temperatures.
The surface loop containing residues 79–83 adopts alternative conformations at low temperatures (top row) but not at high temperatures (bottom two rows). The secondary loop conformation is separated from the body of the protein by an ordered water molecule (red sphere); the van der Waals interactions between the loop and the water may reflect an enthalpic stabilization that is more dominant at low temperatures. The electron density peak to the right of the water corresponds to the backbone carbonyl oxygen of Glu81. 2mFo-DFc electron density contoured at 0.6σ (cyan mesh) and 2.0σ (dark blue mesh). XFEL: X-ray-free electron laser. DOI: http://dx.doi.org/10.7554/eLife.07574.015
Figure 4—figure supplement 1.
Figure 4—figure supplement 1.. Alternative loop conformations are not present in the highest-resolution (1.2 Å) dataset.
The surface loop containing residues 79–83 does not adopt alternative conformations in the 1.2 Å synchrotron dataset. 2mFo-DFc electron density contoured at 0.6σ (cyan mesh) and 2.0σ (dark blue mesh). DOI: http://dx.doi.org/10.7554/eLife.07574.016
Figure 5.
Figure 5.. Quantifying temperature titration of conformational heterogeneity in multiconformer models.
(A) 2mFo-DFc electron density was summed over the volume occupied by the minor conformation but not the major conformation (blue grid points) for Ser99 and Phe113. (B,C) Minor-state 2mFo-DFc electron density increases with temperature. Electron density sums were normalized for each residue. Multitemperature points from synchrotron data are shown in colors corresponding to temperature. The X-ray-free electron laser point is shown as a gray triangle. Best-fit lines are shown for 180 K and below (blue) versus 240 K and above (red). DOI: http://dx.doi.org/10.7554/eLife.07574.018
Figure 6.
Figure 6.. Diversity in temperature dependences of side chain disorder across cyclophilin A (CypA) does not predict the observed average arrest of disorder.
(A) The complement of B-factor-influenced side chain order parameter for the bond most closely associated with the χ1 dihedral angle for Phe113. Lines reflect least-squares fits to synchrotron models at 180 K and below (blue) versus 240 K and above (red). Multitemperature synchrotron points in colors; X-ray-free electron laser (XFEL) point (not included in fits) as gray triangle. (B) Distribution of the intersection temperature between the <200 and >200 K lines fitted with kernel density function. The peak is near 250 K, although there is a tail toward lower temperatures. Intersection temperatures were <170 K for four residues and >330 K for five residues. (C) Predicted and observed values for the complement of side chain order parameter, averaged over all residues in CypA. The predicted values were obtained by extrapolating each residue’s fit line for 240 K and above (red curve) or for the full 100–300 K (purple curve), flooring the result to 0, then averaging across all residues in CypA. Observed values, similarly floored and averaged, are shown as points. DOI: http://dx.doi.org/10.7554/eLife.07574.019
Figure 6—figure supplement 1.
Figure 6—figure supplement 1.. Heterogeneous response of side chain disorder to temperature.
The complement of B-factor-influenced side chain order parameter for the bond most closely associated with the χ1 dihedral angle for all residues in CypA. Lines reflect least-squares fits to synchrotron models for 180 K and below (blue) versus 240 K and above (red). DOI: http://dx.doi.org/10.7554/eLife.07574.020
Figure 6—figure supplement 2.
Figure 6—figure supplement 2.. Residues with persistent disorder across temperatures are interconnected in the crystal lattice.
Several residues with high 1 – S2 values (Val2, Glu15, Gly80, Glu81, Lys82, Pro105, Ala117, Glu120, Lys125, Met142, Ser147, and Lys151) are shown for the central molecule (blue-to-red sticks and backbone) and also in symmetry mates (green sticks, gray backbone). Many of these residues appear to interact with each other via lattice contacts. Frustration in these interactions may lead to persistent disorder. DOI: http://dx.doi.org/10.7554/eLife.07574.021
Figure 6—figure supplement 3.
Figure 6—figure supplement 3.. Diversity in temperature dependences of side chain-end disorder across CypA does not predict the observed average arrest of disorder.
Each panel is as in Figure 6 , but the order parameter now models the final heavy-atom to heavy-atom bond for each side chain (see ‘Methods’). DOI: http://dx.doi.org/10.7554/eLife.07574.022
Figure 6—figure supplement 4.
Figure 6—figure supplement 4.. Heterogeneous response of side chain-end disorder to temperature.
The small multiple plots are as in Figure 6—figure supplement 1 , but the order parameter now models the final heavy-atom to heavy-atom bond for each side chain (see ‘Methods’). DOI: http://dx.doi.org/10.7554/eLife.07574.023
Figure 6—figure supplement 5.
Figure 6—figure supplement 5.. Different residues extrapolate to different maximal-order temperatures.
Fit lines for temperature points at 240 K and above were used to extrapolate to the maximal-order temperature, at which 1 − S2 = 0. (A) Order parameter modeling the bond most closely associated with the χ1 dihedral angle. (B) Order parameter modeling the final heavy-atom to heavy-atom bond for each side chain. DOI: http://dx.doi.org/10.7554/eLife.07574.024
Figure 6—figure supplement 6.
Figure 6—figure supplement 6.. Both harmonic and non-harmonic flexibilities contribute to changes in order parameters with temperature.
(A– D) Contributions to χ1 order parameter versus temperature for four representative residues in CypA. Ser99 and Phe113 are in the active-site network, Glu81 is surface-exposed and adopts alternative conformations at all temperatures (Figure 4), and Phe8 is buried in the protein core and is single-rotamer at all temperatures. Similar conformations within the same rotameric well were grouped together for this analysis. (A) Occupancy of minor alternative conformations. (B) Intra-residue heavy-atom-average B-factor. (C) Complement of the S2ang component of the χ1 order parameter, which uses occupancy-weighted angles between bond vectors across alternative conformations. (D) Complement of the S2ortho component of the χ1 order parameter, which uses occupancy-weighted B-factors. Placement of XFEL points and coloring as in Figure 6A . DOI: http://dx.doi.org/10.7554/eLife.07574.025
Figure 6—figure supplement 7.
Figure 6—figure supplement 7.. Globally averaged disorder exhibits an apparent transition near 250 K.
Points indicate observed values for the complement of side chain order parameters, floored at 0 and then averaged over all residues in CypA, as in Figure 6 . The blue and red lines represent fits to the ≤180 K and ≥240 K floored and averaged points, respectively. The fits to these globally averaged data suggest a transition at ∼250 K, even though the underlying heterogeneity of the individual residue responses does not indicate there is a transition near this temperature. DOI: http://dx.doi.org/10.7554/eLife.07574.026
Figure 7.
Figure 7.. The temperature dependence of side chain disorder is non-homogenously spatially distributed in CypA.
Intersection temperatures from (A) χ1 order parameters as in Figure 6B or (B) side chain terminus order parameters as in Figure 6—figure supplement 3 B are mapped to the 1.2 Å room-temperature synchrotron model. Each residue is marked with a sphere colored based on its apparent transition temperature, from low (blue) to high (red). The active-site network is subdivided: Ser99 and Phe113 (left of boxed region) both transition at a low temperature regardless of order parameter bond vector, but Met61 and Arg55 transition at higher, different temperatures. DOI: http://dx.doi.org/10.7554/eLife.07574.027
Figure 8.
Figure 8.. The dynamic active-site network of cyclophilin A (CypA) has a complex energy landscape.
(A) The previous simple model in which Ser99, Phe113, and Arg55 (Met61 omitted for clarity) interconvert from one macrostate (blue) to the other (red) completely collectively. NMR data suggest this process occurs on a millisecond timescale. (B) A more nuanced model in which network microstates are populated differently depending on the network macrostate, defined by the Phe113 rotameric state. In the left macrostate, Ser99 rotamer changes are disfavored because of steric overlaps with Phe113, but Arg55 rotamer changes are accommodated; the reverse is true (perhaps to a lesser extent) in the right macrostate. Within each microstate, rapid thermal motions occur (bottom right), and may alleviate some minor steric overlaps. Timescales are estimates consistent with NMR observables for CypA and other systems. ms: millisecond; ns: nanosecond; ps: picosecond. DOI: http://dx.doi.org/10.7554/eLife.07574.029

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References

    1. Alcorn T, Juers DH. Progress in rational methods of cryoprotection in macromolecular crystallography. Acta Crystallographica Section D Biological Crystallography. 2010;66:366–373. doi: 10.1107/S090744490903995X. - DOI - PMC - PubMed
    1. Baldwin AJ, Kay LE. NMR spectroscopy brings invisible protein states into focus. Nature Chemical Biology. 2009;5:808–814. doi: 10.1038/nchembio.238. - DOI - PubMed
    1. Burnley BT, Afonine PV, Adams PD, Gros P. Modelling dynamics in protein crystal structures by ensemble refinement. eLife. 2012;1:e00311. doi: 10.7554/eLife.00311. - DOI - PMC - PubMed
    1. Butterfoss GL, Richardson JS, Hermans J. Protein imperfections: separating intrinsic from extrinsic variation of torsion angles. Acta Crystallographica Section D Biological Crystallography. 2005;61:88–98. doi: 10.1107/S0907444904027325. - DOI - PubMed
    1. Chinte U, Shah B, Chen YS, Pinkerton AA, Schall CA, Hanson BL. Cryogenic (<20 K) helium cooling mitigates radiation damage to protein crystals. Acta Crystallographica Section D, Biological Crystallography. 2007;63:486–492. doi: 10.1107/S0907444907005264. - DOI - PubMed

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