SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy

Nat Commun. 2015 Oct 1:6:8497. doi: 10.1038/ncomms9497.


Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR-Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy. The combination of these properties makes this probe a powerful tool for live-cell imaging.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • DNA / chemistry*
  • Drosophila
  • Fluorescent Dyes / chemistry*
  • Humans
  • Microscopy / methods
  • Microscopy, Fluorescence
  • Molecular Imaging / methods
  • Molecular Structure
  • Staining and Labeling / methods


  • Fluorescent Dyes
  • DNA