Electron Tomography Methods for C. elegans

Methods Mol Biol. 2015;1327:141-58. doi: 10.1007/978-1-4939-2842-2_11.

Abstract

Methods for electron tomography of the nematode C. elegans are explained in detail, including a brief introduction to specimen preparation, methods for image collection, and a comparison of several general methods for producing dual-axis tomograms, with or without external fiducial reference objects. New electron tomograms highlight features in software for data display, annotation, and analysis. This chapter discusses the ultrastructural analysis of cells and tissues, rather than molecular studies.

Keywords: Annotation; Back-projection; Denoising; Electron tomogram; Fiducial marker; High pressure freezing; Image acquisition; Image alignment; Markerless alignment.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caenorhabditis elegans / ultrastructure*
  • Electron Microscope Tomography / methods*
  • Image Processing, Computer-Assisted
  • Software