A Method for Obtaining Large Populations of Synchronized Caenorhabditis elegans Dauer Larvae

Methods Mol Biol. 2015;1327:209-19. doi: 10.1007/978-1-4939-2842-2_15.

Abstract

The C. elegans dauer is an attractive model with which to investigate fundamental biological questions, such as how environmental cues are sensed and are translated into developmental decisions through a series of signaling cascades that ultimately result in a transformed animal. Here we describe a simple method of using egg white plates to obtain highly synchronized purified dauers that can be used in downstream applications requiring large quantities of dauers or postdauer animals.

Keywords: Caenorhabditis elegans; Dauer; Diapause; Egg white plate; Pheromone; Postdauer; Stress.

MeSH terms

  • Animals
  • Caenorhabditis elegans / physiology*
  • Larva