Improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a synthetic-peptide 24-mer repeat sequence of glycoprotein G as an antigen

J Vet Med Sci. 2016 Feb;78(2):309-11. doi: 10.1292/jvms.15-0275. Epub 2015 Oct 1.

Abstract

To increase the sensitivity of an enzyme-linked immunosorbent assay (ELISA) for equine herpesvirus type 4 (EHV-4) that uses a 12-mer peptide of glycoprotein G (gG4-12-mer: MKNNPIYSEGSL) [4], we used a longer peptide consisting of a 24-mer repeat sequence (gG4-24-mer: MKNNPIYSEGSLMLNVQHDDSIHT) as an antigen. Sera of horses experimentally infected with EHV-4 reacted much more strongly to the gG4-24-mer peptide than to the gG4-12-mer peptide. We used peptide ELISAs to test paired sera from horses naturally infected with EHV-4 (n=40). gG4-24-mer ELISA detected 37 positive samples (92.5%), whereas gG4-12-mer ELISA detected only 28 (70.0%). gG4-24-mer ELISA was much more sensitive than gG4-12-mer ELISA.

Publication types

  • Evaluation Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Viral / immunology*
  • Chemistry Techniques, Synthetic
  • Enzyme-Linked Immunosorbent Assay / methods
  • Enzyme-Linked Immunosorbent Assay / veterinary*
  • Herpesvirus 4, Equid / isolation & purification*
  • Horse Diseases / virology*
  • Horses
  • Peptide Fragments / chemical synthesis
  • Peptide Fragments / immunology
  • Sensitivity and Specificity
  • Viral Envelope Proteins / chemical synthesis
  • Viral Envelope Proteins / immunology*

Substances

  • Antigens, Viral
  • Peptide Fragments
  • Viral Envelope Proteins
  • glycoprotein G, Equid herpesvirus 4