Abstract
LEAFY COTYLEDON1 (LEC1) is a B subunit of Nuclear Factor Y (NF-YB) transcription factor that mainly accumulates during embryo development. We cloned the 5' flanking regulatory sequence of AhLEC1B gene, a homolog of Arabidopsis LEC1, and analyzed its regulatory elements using online software. To identify the crucial regulatory region, we generated a series of GUS expression frameworks driven by different length promoters with 5' terminal and/or 3' terminal deletion. We further characterized the GUS expression patterns in the transgenic Arabidopsis lines. Our results show that both the 65 bp proximal promoter region and the 52 bp 5' UTR of AhLEC1B contain the key motifs required for the essential promoting activity. Moreover, AhLEC1B is preferentially expressed in the embryo and is co-regulated by binding of its upstream genes with both positive and negative corresponding cis-regulatory elements.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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5' Flanking Region / genetics*
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Arabidopsis / genetics*
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Arabidopsis / growth & development
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Arabidopsis / metabolism
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Arachis / genetics*
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Arachis / growth & development
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Arachis / metabolism
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Base Sequence
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Cloning, Molecular
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Gene Expression Regulation, Plant*
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Molecular Sequence Data
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Phylogeny
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Plant Proteins / genetics*
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Plants, Genetically Modified / genetics*
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Plants, Genetically Modified / growth & development
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Plants, Genetically Modified / metabolism
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Promoter Regions, Genetic / genetics
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RNA, Plant / genetics
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Regulatory Sequences, Nucleic Acid / genetics*
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Transcription Factors / metabolism
Substances
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Plant Proteins
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RNA, Plant
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Transcription Factors
Grants and funding
This work was funded by National Natural Science Foundation of China, grant No. 30971546 and No. 31470349, to LS, and No. 31171621 to ZJL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.