The zebrafish galectins Drgal1-L2 and Drgal3-L1 bind in vitro to the infectious hematopoietic necrosis virus (IHNV) glycoprotein and reduce viral adhesion to fish epithelial cells

Dev Comp Immunol. 2016 Feb;55:241-252. doi: 10.1016/j.dci.2015.09.007. Epub 2015 Sep 30.

Abstract

The infectious hematopoietic necrosis virus (IHNV; Rhabdoviridae, Novirhabdovirus) infects teleost fish, such as salmon and trout, and is responsible for significant losses in the aquaculture industry and in wild fish populations. Although IHNV enters the host through the skin at the base of the fins, the viral adhesion and entry mechanisms are not fully understood. In recent years, evidence has accumulated in support of the key roles played by protein-carbohydrate interactions between host lectins secreted to the extracellular space and virion envelope glycoproteins in modulating viral adhesion and infectivity. In this study, we assessed in vitro the potential role(s) of zebrafish (Danio rerio) proto type galectin-1 (Drgal1-L2) and a chimera galectin-3 (Drgal3-L1) in IHNV adhesion to epithelial cells. Our results suggest that the extracellular Drgal1-L2 and Drgal3-L1 interact directly and in a carbohydrate-dependent manner with the IHNV glycosylated envelope and glycans on the epithelial cell surface, significantly reducing viral adhesion.

Keywords: Epithelial cells; Galectin; IHNV envelope glycoprotein; Infectious hematopoietic necrosis virus (IHNV); Viral adhesion; Zebrafish.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Epithelial Cells / physiology*
  • Epithelial Cells / virology
  • Galectins / genetics
  • Galectins / metabolism*
  • Host-Pathogen Interactions
  • Infectious hematopoietic necrosis virus / immunology*
  • Infectious hematopoietic necrosis virus / pathogenicity
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism*
  • Rhabdoviridae Infections / immunology*
  • Rhabdoviridae Infections / transmission
  • Viral Envelope Proteins / metabolism*
  • Virulence
  • Virus Attachment
  • Zebrafish / immunology*
  • Zebrafish Proteins / genetics
  • Zebrafish Proteins / metabolism*

Substances

  • Gal1-L2 protein, zebrafish
  • Galectins
  • Recombinant Fusion Proteins
  • Viral Envelope Proteins
  • Zebrafish Proteins