Purification and Injection of Retroviral Vectors

Cold Spring Harb Protoc. 2015 Oct 1;2015(10):925-31. doi: 10.1101/pdb.prot086371.


Retroviral vectors are powerful tools for genetic manipulation. This protocol discusses the production, purification, and use of replication-deficient retroviral vectors based on Moloney murine leukemia virus and lentivirus. It also describes the injection of a retroviral vector into the dentate gyrus of young adult mice to fluorescently label live murine brain tissue.

MeSH terms

  • Animals
  • Genetic Vectors / isolation & purification*
  • Lentivirus / genetics
  • Lentivirus / growth & development*
  • Lentivirus / isolation & purification*
  • Mice
  • Moloney murine leukemia virus / genetics
  • Moloney murine leukemia virus / growth & development*
  • Moloney murine leukemia virus / isolation & purification*
  • Virus Replication