Imaging Newborn Granule Cells in Fixed Sections

Cold Spring Harb Protoc. 2015 Oct 1;2015(10):932-3. doi: 10.1101/pdb.prot086389.


This protocol describes the harvesting of brain tissue from mice that have had the retroviral vector CAG-GFP injected into the dentate gyrus. Brain tissue from these mice is dissected, the tissue is fixed, and the sections are prepared. The fixed sections are imaged using fluorescent confocal microscopy, and newborn granule cells containing GFP are visualized and are characterized.

MeSH terms

  • Animals
  • Animals, Newborn
  • Brain / cytology*
  • Green Fluorescent Proteins / analysis
  • Green Fluorescent Proteins / genetics
  • Mice
  • Microscopy, Confocal / methods*
  • Microtomy
  • Neurons / cytology*
  • Staining and Labeling / methods
  • Tissue Fixation


  • Green Fluorescent Proteins