Analysis of Spine Motility of Newborn Granule Cells in Acute Brain Slices

Cold Spring Harb Protoc. 2015 Oct 1;2015(10):934-6. doi: 10.1101/pdb.prot086397.


In this protocol, acute brain slices are prepared from mice in which newborn granule cells have been labeled using retroviral vector technology. Using a live-cell imaging stage and confocal microscopy coupled to imaging software, dendritic spines are analyzed.

MeSH terms

  • Animals
  • Brain / cytology*
  • Dendritic Spines / physiology*
  • Humans
  • Image Processing, Computer-Assisted
  • Infant, Newborn
  • Mice
  • Microscopy, Confocal / methods
  • Neuronal Plasticity*
  • Neurons / cytology*
  • Spine / cytology*
  • Staining and Labeling / methods