Preparation and Use of Retroviral Vectors for Labeling, Imaging, and Genetically Manipulating Cells

Cold Spring Harb Protoc. 2015 Oct 1;2015(10):883-8. doi: 10.1101/pdb.top086363.


Retroviral vectors are a powerful technology for achieving long-term genetic manipulation. This introduction provides some background on replication-deficient retroviral vectors based on Moloney murine leukemia virus and lentivirus. Details, examples, and associated protocols are provided for using these vectors to fluorescently label, genetically alter, and image both live and fixed murine brain tissue.

MeSH terms

  • Animals
  • Brain / cytology
  • Genetic Vectors / isolation & purification*
  • Lentivirus / genetics
  • Lentivirus / growth & development*
  • Lentivirus / isolation & purification*
  • Mice
  • Moloney murine leukemia virus / genetics
  • Moloney murine leukemia virus / growth & development*
  • Moloney murine leukemia virus / isolation & purification*
  • Staining and Labeling / methods
  • Transformation, Genetic
  • Virus Replication