LAMTOR1-PRKCD and NUMA1-SFMBT1 fusion genes identified by RNA sequencing in aneurysmal benign fibrous histiocytoma with t(3;11)(p21;q13)

Cancer Genet. 2015 Nov;208(11):545-51. doi: 10.1016/j.cancergen.2015.07.007. Epub 2015 Aug 3.


RNA sequencing of an aneurysmal benign fibrous histiocytoma with the karyotype 46,XY,t(3;11)(p21;q13),del(6)(p23)[17]/46,XY[2] showed that the t(3;11) generated two fusion genes: LAMTOR1-PRKCD and NUMA1-SFMBT1. RT-PCR together with Sanger sequencing verified the presence of fusion transcripts from both fusion genes. In the LAMTOR1-PRKCD fusion, the part of the PRKCD gene coding for the catalytic domain of the serine/threonine kinase is under control of the LAMTOR1 promoter. In the NUMA1-SFMBT1 fusion, the part of the SFMBT1 gene coding for two of four malignant brain tumor domains and the sterile alpha motif domain is controlled by the NUMA1 promoter. The data support a neoplastic genesis of aneurysmal benign fibrous histiocytoma and indicate a pathogenetic role for LAMTOR1-PRKCD and NUMA1-SFMBT1.

Keywords: Aneurysmal benign fibrous histiocytoma; LAMTOR1-PRKCD; NUMA1-SFMBT1; RNA sequencing; cytogenetics; fusion genes.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aneurysm / genetics*
  • Aneurysm / pathology
  • Antigens, Nuclear / genetics
  • Carrier Proteins / genetics
  • Cell Cycle Proteins
  • Chromosomes, Human, Pair 11 / genetics
  • Chromosomes, Human, Pair 3 / genetics
  • Histiocytoma, Benign Fibrous / genetics*
  • Histiocytoma, Benign Fibrous / pathology
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Male
  • Nuclear Matrix-Associated Proteins / genetics
  • Oncogene Fusion*
  • Protein Kinase C-delta / genetics
  • Repressor Proteins / genetics
  • Sequence Analysis, RNA / methods*
  • Translocation, Genetic*


  • Antigens, Nuclear
  • Carrier Proteins
  • Cell Cycle Proteins
  • Intracellular Signaling Peptides and Proteins
  • LAMTOR1 protein, human
  • NUMA1 protein, human
  • Nuclear Matrix-Associated Proteins
  • Repressor Proteins
  • SFMBT1 protein, human
  • PRKCD protein, human
  • Protein Kinase C-delta