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, 20 (1), 26-32

Chronic Aerobic Exercise Decreases Lectin-Like Low Density Lipoprotein (LOX-1) Receptor Expression in Heart of Diabetic Rat

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Chronic Aerobic Exercise Decreases Lectin-Like Low Density Lipoprotein (LOX-1) Receptor Expression in Heart of Diabetic Rat

Simin Riahi et al. Iran Biomed J.

Abstract

Background: Overexpression of lectin-like low density lipoprotein (LOX-1) receptor plays an important role in hyperglycemia-induced vascular complications such as atherosclerosis. Based on the beneficial effects of exercise on preventing cardiovascular complications of diabetes, we aimed to examine the protective effects of aerobic exercise on expression of LOX-1 receptor and production of free radicals in the heart of diabetic rats.

Methods: Four groups of rats were used: (n = 5 per group): sedentary normal, trained normal, sedentary diabetes and trained diabetes. Diabetes was induced by a single intraperitoneal injection of streptozotocin (50 mg/kg). The exercise protocol was consisted of swimming 30 min/day, 5 days/week for eight weeks. Plasma glucose was evaluated at initiation, weeks 4 and 8 of experiment. At the end of experiment, rats were sacrificed and the heart was removed for determination of nitrate, malondialdehyde, and LOX-1 gene expression.

Results: In normal non-diabetic rats, the blood glucose level was <150 mg/dl; however, the induction of diabetes resulted in levels more than >400 mg/dl. Gene expression of LOX-1 was increased in the heart of diabetic rats. Exercise reduced the gene expression of this protein in diabetic states without reducing the blood glucose. Finally, swimming exercise decreased the malondialdehyde and nitrate levels in heart tissue both in control and diabetic rats.

Conclusion: Swimming exercise reduces heart expression of the LOX-1 receptor in accompany with reduction of free radicals production. Since these parameters are important in generation of diabetic complications, swimming exercise is a good candidate for reducing these complications.

Keywords: Diabetic complications; Exercise; Free radicals; Hyperglycemia; LOX-1 receptor.

Figures

Fig. 1.
Fig. 1.
mRNA of lectin-like oxidized low-density lipoprotein (LOX-1) receptor in cardiac cells of different experimental groups (left hand). RT-PCR products were visualized on agarose gel (1.5%) using ethidium bromide staining method. The graph shows the density of LOX-1 gene that was normalized relative to density of β-Actin gene for different groups (right hand). All values are presented as mean ± SEM. *Significantly different from sedentary normal group (P<0.05); #Significantly different from sedentary diabetic group (P<0.05
Fig. 2
Fig. 2
Effect of eight-week swimming exercise on malondialdehyde (MDA) content in cardiac tissue. MDA values are indicated as nmol/mg protein. All values are presented as mean ± SEM. *Significantly different from sedentary normal group (P<0.05); #Significantly different from sedentary diabetic group (P<0.001
Fig. 3
Fig. 3
Effect of eight-week swimming exercise on nitrate (NOx) content in cardiac tissue. NOx values are indicated as nmol/mg protein. All values are presented as mean ± SEM. *Significantly different from sedentary normal group (P<0.05); #Significantly different from sedentary diabetic group (P<0.01

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