Inhibition of Aldose Reductase and Anti-Cataract Action of Trans-Anethole Isolated From Foeniculum Vulgare Mill. Fruits

Food Chem. 2012 May 1;132(1):385-90. doi: 10.1016/j.foodchem.2011.11.005. Epub 2011 Nov 10.

Abstract

Foeniculum vulgare fruits are routinely consumed for their carminative and mouth freshening effect. The plant was evaluated for aldose reductase inhibition and anti-diabetic action. Bioguided fractionation using silica gel column chromatography, HPLC, and GC-MS analysis revealed trans-anethole as the bioactive constituent possessing potent aldose reductase inhibitory action, with an IC50 value of 3.8μg/ml. Prolonged treatment with the pet ether fraction of the F. vulgare distillate demonstrated improvement in blood glucose, lipid profile, glycated haemoglobin and other parameters in streptozotocin-induced diabetic rats. Trans-anethole could effectively show anti-cataract activity through the increase in soluble lens protein, reduced glutathione, catalase and SOD activity on in vitro incubation of the eye lens with 55mM glucose. Trans-anethole demonstrated noncompetitive to mixed type of inhibition of lens aldose reductase using Lineweaver Burk plot.

Keywords: Aldose reductase inhibitor; Anti-cataract; Antidiabetic; Foeniculum vulgare; Trans-anethole.

MeSH terms

  • Aldehyde Reductase / antagonists & inhibitors*
  • Animals
  • Anisoles / chemistry*
  • Cataract / prevention & control*
  • Diabetes Mellitus, Experimental / drug therapy*
  • Foeniculum / chemistry*
  • Fruit / chemistry*
  • Male
  • Plant Extracts / chemistry*
  • Rats
  • Rats, Wistar
  • Streptozocin / adverse effects*

Substances

  • Anisoles
  • Plant Extracts
  • Streptozocin
  • Aldehyde Reductase
  • anethole