Monoclonal antibody profiling of cell surface proteins associated with the viral biofilms on HTLV-1 transformed cells

Acta Virol. 2015 Sep;59(3):247-56. doi: 10.4149/av_2015_03_247.

Abstract

Human T lymphotropic virus 1 (HTLV-1) is a pathogenic retrovirus that spreads predominantly via cell-to-cell contact. Two models of cell-to-cell virus transmission are proposed: virological synapse (VS) and viral biofilms (VB). Both infectious structures can be involved in transmission and synergistically enhance HTLV-1 spread between cells. Although transmission of virus via VB has been reported, the molecular composition of VB remains poorly understood. In this study we generated new anti-VB monoclonal antibodies (MAbs) and screenedthem along with a panel of anti-human cluster of differentiation (CD) MAbs to select antigens associated with VB. Among four MAbs generated against VB, two MAbs were identified as anti-CD25 (IL-2RA). We found that antigens CD4, CD150, CD25, CD70, and CD80 were enriched in VB. We also determined that expression of viral protein Tax, a central molecule in HTLV-1 transmission, upregulates intercellular adhesion molecule 1 (ICAM-1), CD95, CD25, CD70, and CD80. Whether these antigens are essential for VB formation and HTLV-1 infection remains unknown and will be determined in further experiments.

Keywords: monoclonal antibody; CD antigen; HTLV-1; biofilms..

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / biosynthesis*
  • Biofilms*
  • Cell Transformation, Viral
  • Genes, pX / physiology
  • HEK293 Cells
  • HTLV-I Infections / transmission*
  • Human T-lymphotropic virus 1 / immunology*
  • Humans

Substances

  • Antibodies, Monoclonal