Mechanism and Regulation of Cytoplasmic Dynein

Annu Rev Cell Dev Biol. 2015;31:83-108. doi: 10.1146/annurev-cellbio-100814-125438. Epub 2015 Sep 30.

Abstract

Until recently, dynein was the least understood of the cytoskeletal motors. However, a wealth of new structural, mechanistic, and cell biological data is shedding light on how this complicated minus-end-directed, microtubule-based motor works. Cytoplasmic dynein-1 performs a wide array of functions in most eukaryotes, both in interphase, in which it transports organelles, proteins, mRNAs, and viruses, and in mitosis and meiosis. Mutations in dynein or its regulators are linked to neurodevelopmental and neurodegenerative diseases. Here, we begin by providing a synthesis of recent data to describe the current model of dynein's mechanochemical cycle. Next, we discuss regulators of dynein, with particular focus on those that directly interact with the motor to modulate its recruitment to microtubules, initiate cargo transport, or activate minus-end-directed motility.

Keywords: Lis1; Nudel/NudE; cytoskeleton; dynactin; microtubule; transport.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Biological Transport / physiology
  • Cytoplasmic Dyneins / metabolism*
  • Humans
  • Meiosis / physiology
  • Microtubule-Associated Proteins / metabolism
  • Microtubules / metabolism
  • Mitosis / physiology
  • Organelles / metabolism
  • Organelles / physiology

Substances

  • Microtubule-Associated Proteins
  • Cytoplasmic Dyneins