Interleukin-23 Facilitates Thyroid Cancer Cell Migration and Invasion by Inhibiting SOCS4 Expression via MicroRNA-25

Zhidan Mei; Shiming Chen; Chen Chen; Bokui Xiao; Fen Li; Yongping Wang; Zezhang Tao

doi:10.1371/journal.pone.0139456

Interleukin-23 Facilitates Thyroid Cancer Cell Migration and Invasion by Inhibiting SOCS4 Expression via MicroRNA-25

PLoS One. 2015 Oct 5;10(10):e0139456. doi: 10.1371/journal.pone.0139456. eCollection 2015.

Authors

Zhidan Mei¹, Shiming Chen¹, Chen Chen², Bokui Xiao², Fen Li², Yongping Wang¹, Zezhang Tao³

Affiliations

¹ Department of Otolaryngology Head and Neck Surgery, Remin Hospital of Wuhan University, Wuhan, 430060, Hubei Province, the People's Republic of China.
² Research Institute of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei Province, the People's Republic of China.
³ Department of Otolaryngology Head and Neck Surgery, Remin Hospital of Wuhan University, Wuhan, 430060, Hubei Province, the People's Republic of China; Research Institute of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei Province, the People's Republic of China.

Abstract

Interleukin-23 (IL-23) is a conventional proinflammatory cytokine that plays a role in tumor progression by inducing inflammation in the tumor microenvironment. However, the role of IL-23 in thyroid cancer migration and invasion remains unclear. In the present study, we observed that the treatment with IL-23, induced migration and invasion in human thyroid cancer cells. Additional data demonstrate that SOCS4 negatively regulates IL-23-mediated migration and invasion. On investigating the mechanisms involved in IL-23 mediated migration and invasion, we observed that miR-25 promotes the migration and invasion of thyroid cancer cells by directly binding to the 3'-UTR of SOCS4 that leads to the inhibition of SOCS4. In addition, we also demonstrated that IL-23 increases miR-25 expression levels, and overexpressed miR-25 is involved in IL-23-associated SOCS4 inhibition and cell migration and invasion. Together, our data suggest that IL-23 induces migration and invasion in thyroid cancer cells by mediating the miR-25/SOCS4 signaling pathway.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3' Untranslated Regions
Adult
Cell Movement / drug effects
DNA, Neoplasm / metabolism
Female
Gene Expression Regulation, Neoplastic / genetics
Gene Expression Regulation, Neoplastic / physiology
Humans
Interleukin-23 / pharmacology*
Male
MicroRNAs / physiology*
Middle Aged
Neoplasm Invasiveness / physiopathology*
RNA, Small Interfering / genetics
Real-Time Polymerase Chain Reaction
Signal Transduction
Suppressor of Cytokine Signaling Proteins / biosynthesis
Suppressor of Cytokine Signaling Proteins / genetics
Suppressor of Cytokine Signaling Proteins / physiology*
Thyroid Neoplasms / pathology*
Tumor Cells, Cultured
Up-Regulation

Substances

3' Untranslated Regions
DNA, Neoplasm
Interleukin-23
MIRN25 microRNA, human
MicroRNAs
RNA, Small Interfering
SOCS4 protein, human
Suppressor of Cytokine Signaling Proteins

Grants and funding

This work was supported by the grants from the National Natural Science Foundation of China (No.81372880); the Independent research project of Wuhan University (No. 2042014kf0184; NO. 2042014kf0119); the doctoral program of Higher Education Research Fund (No. 20130141120093; No. 20110141110062); the Natural Science Foundation of Hubei province (No.2012FFA045). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.