New methodologies. Their role in pediatric pathology

Pediatr Clin North Am. 1989 Feb;36(1):227-56. doi: 10.1016/s0031-3955(16)36624-x.


Recombinant DNA technology is providing the means for early and specific etiologic diagnoses of infectious and immunologic diseases, replacing or complementing older methodologies. The new tools that have been so useful in detecting gene rearrangements in leukemias and lymphomas are being applied to the unresolved questions of embryogenesis and disorderly cell differentiation and are being used to completely re-map the nervous system. Flow cytometry and cell sorting are becoming standard features of clinical laboratories and are instrumental not only in defining alterations in lymphoid cell populations but in examining cellular functions as well as surface markers. Bone marrow and organ transplantation for genetic, metabolic, and neoplastic diseases will be performed much more effectively as these newer technologies are applied to the selection of compatible donors and to the follow-up of rejection and infectious complications.

Publication types

  • Case Reports
  • Review

MeSH terms

  • Adolescent
  • Cell Separation*
  • Cytokinins / analysis
  • Female
  • Flow Cytometry*
  • Humans
  • Immune System Diseases / diagnosis*
  • Infections / diagnosis*
  • Male
  • Nucleic Acid Probes*
  • Pediatrics
  • Transplantation


  • Cytokinins
  • Nucleic Acid Probes