Halorubrum rutilum sp. nov. isolated from a marine solar saltern

Arch Microbiol. 2015 Dec;197(10):1159-64. doi: 10.1007/s00203-015-1159-3. Epub 2015 Oct 5.


A halophilic archaeal strain, YJ-18-S1(T), was isolated from Yangjiang marine solar saltern, Guangxi Province, China. Cells were pleomorphic, stained Gram-negative and formed red-pigmented colonies on agar plates. Strain YJ-18-S1(T) was able to grow at 20-55 °C (optimum 37 °C), at 0.9-4.8 M NaCl (optimum 2.6 M NaCl), at 0.005-1.0 M MgCl2 (optimum 0.3 MgCl2) and at pH 5.5-8.5 (optimum pH 7.0). The cells were lysed in distilled water, and the minimal NaCl concentration to prevent cell lysis was found to be 5 % (w/v). The major polar lipids of the strain were phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and sulfated mannosyl glucosyl diether. The 16S rRNA gene and rpoB' gene of strain YJ-18-S1(T) were phylogenetically related to the corresponding genes of Halorubrum members (94.3-98.0 and 86.7-96.1 % similarities, respectively). The DNA G+C content of strain YJ-18-S1(T) was 66.2 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain YJ-18-S1(T) (=CGMCC 1.12554(T) = JCM 30030(T)) represents a new species of Halorubrum, for which the name Halorubrum rutilum sp. nov. is proposed.

Keywords: Halophilic archaeon; Halorubrum rutilum sp. nov.; Marine solar saltern.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Composition / genetics
  • China
  • DNA, Archaeal / genetics
  • Halorubrum* / classification
  • Halorubrum* / genetics
  • Halorubrum* / isolation & purification
  • Phospholipids / genetics*
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Seawater / microbiology*
  • Sodium Chloride / metabolism*


  • DNA, Archaeal
  • PGP-Me
  • Phospholipids
  • RNA, Ribosomal, 16S
  • Sodium Chloride