SecA protein is directly involved in protein secretion in Escherichia coli

H Kawasaki; S Matsuyama; S Sasaki; M Akita; S Mizushima

doi:10.1016/0014-5793(89)80516-2

SecA protein is directly involved in protein secretion in Escherichia coli

FEBS Lett. 1989 Jan 2;242(2):431-4. doi: 10.1016/0014-5793(89)80516-2.

Authors

H Kawasaki¹, S Matsuyama, S Sasaki, M Akita, S Mizushima

Affiliation

¹ Institute of Applied Microbiology, University of Tokyo, Japan.

PMID: 2644134
DOI: 10.1016/0014-5793(89)80516-2

Abstract

A high-expression plasmid for the secA gene was constructed. The SecA protein was then overproduced in E. coli and purified. The purified SecA stimulated the in vitro translocation of a model secretory protein into inverted membrane vesicles pretreated with 4 M urea. Membrane vesicles from a secAts mutant exhibited lower translocation activity, which was enhanced by SecA. These results indicate that SecA is directly involved in protein secretion across the cytoplasmic membrane.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Outer Membrane Proteins / metabolism
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Cloning, Molecular
Escherichia coli / genetics
Escherichia coli / metabolism*
Genes, Bacterial
In Vitro Techniques
Plasmids

Substances

Bacterial Outer Membrane Proteins
Bacterial Proteins