Cell-to-cell infection by HIV contributes over half of virus infection

Elife. 2015 Oct 6;4:e08150. doi: 10.7554/eLife.08150.

Abstract

Cell-to-cell viral infection, in which viruses spread through contact of infected cell with surrounding uninfected cells, has been considered as a critical mode of virus infection. However, since it is technically difficult to experimentally discriminate the two modes of viral infection, namely cell-free infection and cell-to-cell infection, the quantitative information that underlies cell-to-cell infection has yet to be elucidated, and its impact on virus spread remains unclear. To address this fundamental question in virology, we quantitatively analyzed the dynamics of cell-to-cell and cell-free human immunodeficiency virus type 1 (HIV-1) infections through experimental-mathematical investigation. Our analyses demonstrated that the cell-to-cell infection mode accounts for approximately 60% of viral infection, and this infection mode shortens the generation time of viruses by 0.9 times and increases the viral fitness by 3.9 times. Our results suggest that even a complete block of the cell-free infection would provide only a limited impact on HIV-1 spread.

Keywords: HIV-1; basic reproduction number; cell-free infection; cell-to-cell infection; computational biology; infectious disease; mathematical model; microbiology; systems biology; viruses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • HIV Infections / virology*
  • HIV-1 / physiology*
  • Humans
  • Jurkat Cells
  • Models, Theoretical
  • Virus Internalization*
  • Virus Release*

Grant support

The funder had no role in study design, data collection and interpretation, or the decision to submit the work for publication.