Isolation, Culture, and Polarization of Murine Bone Marrow-Derived and Peritoneal Macrophages

Methods Mol Biol. 2015;1339:101-9. doi: 10.1007/978-1-4939-2929-0_6.


Macrophages are the most specialized phagocytic cells, and acquire specific phenotypes and functions in response to a variety of external triggers. Culture of bone marrow-derived or peritoneal macrophages from mice represents an exceptionally powerful technique to investigate macrophage phenotypes and functions in response to specific stimuli, resembling as much as possible the conditions observed in various pathophysiological settings. This chapter outlines protocols used to isolate and culture murine bone marrow-derived and peritoneal macrophages. Furthermore, we describe how these macrophages can be "polarized" to obtain specific macrophage subsets with special relevance to atherosclerosis.

Keywords: Atherosclerosis; Bone marrow-derived macrophages; Macrophage polarization; Peritoneal macrophages.

MeSH terms

  • Animals
  • Atherosclerosis / immunology
  • Atherosclerosis / metabolism*
  • Atherosclerosis / pathology
  • Cell Differentiation
  • Cell Separation / methods*
  • Cells, Cultured
  • Culture Media / metabolism
  • Cytokines / immunology
  • Cytokines / metabolism
  • Macrophages / immunology
  • Macrophages / metabolism*
  • Macrophages / pathology
  • Macrophages, Peritoneal / immunology
  • Macrophages, Peritoneal / metabolism*
  • Macrophages, Peritoneal / pathology
  • Mice
  • Phenotype
  • Primary Cell Culture*


  • Culture Media
  • Cytokines