ProPepper: a curated database for identification and analysis of peptide and immune-responsive epitope composition of cereal grain protein families

Database (Oxford). 2015 Oct 8;2015:bav100. doi: 10.1093/database/bav100. Print 2015.

Abstract

ProPepper is a database that contains prolamin proteins identified from true grasses (Poaceae), their peptides obtained with single- and multi-enzyme in silico digestions as well as linear T- and B-cell-specific epitopes that are responsible for wheat-related food disorders. The integrated database and analysis platform contains datasets that are collected from multiple public databases (UniprotKB, IEDB, NCBI GenBank), manually curated and annotated, and interpreted in three main data tables: Protein-, Peptide- and Epitope list views that are cross-connected by unique identifications. Altogether 21 genera and 80 different species are represented. Currently, the database contains 2146 unique and complete protein sequences related to 2618 GenBank entries and 35 657 unique peptide sequences that are a result of 575 110 unique digestion events obtained by in silico digestion methods involving six proteolytic enzymes and their combinations. The interface allows advanced global and parametric search functions along with a download option, with direct connections to the relevant public databases. Database URL: https://propepper.net.

Publication types

  • Dataset
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Databases, Protein*
  • Edible Grain* / chemistry
  • Edible Grain* / genetics
  • Edible Grain* / immunology
  • Epitopes, B-Lymphocyte* / chemistry
  • Epitopes, B-Lymphocyte* / genetics
  • Epitopes, B-Lymphocyte* / immunology
  • Epitopes, T-Lymphocyte* / chemistry
  • Epitopes, T-Lymphocyte* / genetics
  • Epitopes, T-Lymphocyte* / immunology
  • Humans
  • Plant Proteins* / chemistry
  • Plant Proteins* / genetics
  • Plant Proteins* / immunology
  • Poaceae* / chemistry
  • Poaceae* / genetics
  • Poaceae* / metabolism

Substances

  • Epitopes, B-Lymphocyte
  • Epitopes, T-Lymphocyte
  • Plant Proteins