Directly Reprogrammed Human Neurons Retain Aging-Associated Transcriptomic Signatures and Reveal Age-Related Nucleocytoplasmic Defects

Cell Stem Cell. 2015 Dec 3;17(6):705-718. doi: 10.1016/j.stem.2015.09.001. Epub 2015 Oct 8.

Abstract

Aging is a major risk factor for many human diseases, and in vitro generation of human neurons is an attractive approach for modeling aging-related brain disorders. However, modeling aging in differentiated human neurons has proved challenging. We generated neurons from human donors across a broad range of ages, either by iPSC-based reprogramming and differentiation or by direct conversion into induced neurons (iNs). While iPSCs and derived neurons did not retain aging-associated gene signatures, iNs displayed age-specific transcriptional profiles and revealed age-associated decreases in the nuclear transport receptor RanBP17. We detected an age-dependent loss of nucleocytoplasmic compartmentalization (NCC) in donor fibroblasts and corresponding iNs and found that reduced RanBP17 impaired NCC in young cells, while iPSC rejuvenation restored NCC in aged cells. These results show that iNs retain important aging-related signatures, thus allowing modeling of the aging process in vitro, and they identify impaired NCC as an important factor in human aging.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Aging*
  • Cell Nucleus / metabolism*
  • Cell Separation
  • Cellular Reprogramming*
  • Child
  • Child, Preschool
  • Cytoplasm / metabolism*
  • Fibroblasts / cytology
  • Flow Cytometry
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Infant
  • Infant, Newborn
  • Middle Aged
  • Neural Cell Adhesion Molecules / metabolism
  • Neurons / cytology*
  • Transcriptome
  • Young Adult
  • ran GTP-Binding Protein / metabolism

Substances

  • Neural Cell Adhesion Molecules
  • RanGTP-binding protein 17
  • ran GTP-Binding Protein