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. 2015;2015:706186.
doi: 10.1155/2015/706186. Epub 2015 Sep 17.

Effect of the Human Amniotic Membrane on Liver Regeneration in Rats

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Free PMC article

Effect of the Human Amniotic Membrane on Liver Regeneration in Rats

Mesut Sipahi et al. HPB Surg. .
Free PMC article

Abstract

Introduction. Operations are performed for broader liver surgery indications for a better understanding of hepatic anatomy/physiology and developments in operation technology. Surgery can cure some patients with liver metastasis of some tumors. Nevertheless, postoperative liver failure is the most feared complication causing mortality in patients who have undergone excision of a large liver mass. The human amniotic membrane has regenerative effects. Thus, we investigated the effects of the human amniotic membrane on regeneration of the resected liver. Methods. Twenty female Wistar albino rats were divided into control and experimental groups and underwent a 70% hepatectomy. The human amniotic membrane was placed over the residual liver in the experimental group. Relative liver weight, histopathological features, and biochemical parameters were assessed on postoperative day 3. Results. Total protein and albumin levels were significantly lower in the experimental group than in the control group. No difference in relative liver weight was observed between the groups. Hepatocyte mitotic count was significantly higher in the experimental group than in the control group. Hepatic steatosis was detected in the experimental group. Conclusion. Applying the amniotic membrane to residual liver adversely affected liver regeneration. However, mesenchymal stem cell research has the potential to accelerate liver regeneration investigations.

Figures

Figure 1
Figure 1
Resected liver in study group.
Figure 2
Figure 2
(a) Microscopic photo illustrating high Ki-67 positivity of a case of control group (Avidin-biotin-peroxidase method, ×200). (b) Microscopic photo illustrating high Ki-67 positivity of an amniotic membrane used case (Avidin-biotin-peroxidase method, ×200).
Figure 3
Figure 3
(a) Ki-67 levels. (b) Relative Liver weights (g).
Figure 4
Figure 4
(a) The biopsy of a case of control group showing no considerable specific pathology (H&E, ×200). (b) The photomicrograph of an amniotic membrane used case showing extensive macrovesicular steatosis (arrow) and a mitotic figure (circle) (H&E, ×400).

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