Extension of resolution and oligomerization-state studies of 2,4'-dihydroxyacetophenone dioxygenase from Alcaligenes sp. 4HAP

Acta Crystallogr F Struct Biol Commun. 2015 Oct;71(Pt 10):1258-63. doi: 10.1107/S2053230X15015873. Epub 2015 Sep 23.


The enzyme 2,4'-dihydroxyacetophenone dioxygenase (DAD) catalyses the conversion of 2,4'-dihydroxyacetophenone to 4-hydroxybenzoic acid and formic acid. This enzyme is a very unusual dioxygenase in that it cleaves a C-C bond in a substituent of the aromatic ring rather than within the ring itself. Whilst it has been shown that DAD is a tetramer in solution, the recently solved crystal structure of the Alcaligenes sp. 4HAP enzyme was in fact dimeric rather than tetrameric. Since the use of limited chymotrypsinolysis, which apparently results in removal of the first 20 or so N-terminal residues of DAD, was necessary for crystallization of the protein, it was investigated whether this was responsible for the change in its oligomerization state. Gel-filtration and analytical ultracentrifugation studies were conducted, which confirmed that chymotrypsinolysed DAD has an apparent molecular weight of around 40 kDa, corresponding to a dimer. In contrast, the native enzyme has a molecular weight in the 70-80 kDa region, as expected for the tetramer. The structural basis for tetramerization has been investigated by the use of several docking servers, and the results are remarkably consistent with the tetrameric structure of a homologous cupin protein from Ralstonia eutropha (PDB entry 3ebr).

Keywords: 2,4′-dihydroxyacetophenone dioxygenase; analytical ultracentrifugation; dioxygenase; docking; gel filtration; oligomerization; protein X-ray structure.

MeSH terms

  • Alcaligenes / enzymology*
  • Biocatalysis
  • Chromatography, Gel
  • Crystallization
  • Crystallography, X-Ray
  • Dioxygenases / chemistry*
  • Models, Molecular
  • Protein Multimerization*
  • Protein Structure, Quaternary
  • Static Electricity
  • Ultracentrifugation


  • Dioxygenases
  • 2,4'-dihydroxyacetophenone dioxygenase

Associated data

  • PDB/5BPX