Background: To evaluate the large quantities of genetically modified soybeans that are imported into China, we evaluated the presence of transgenes in soybean samples from an area, which are major soybean-producing areas. We evaluated the presence of exogenous genes in a total of 254 soybean samples from farms and 35 commercially available soybean samples using polymerase chain reaction amplification and sequence analysis.
Results: The farm soybean samples contained a total of 67 CaMV35S-positive samples, 46 NOS-positive samples, 16 CP4-EPEPS-positive samples, and 66 BT-positive samples. The commercially available soybean samples contained a total of 12 CaMV35S-positive samples, 19 NOS-positive samples, 13 CP4-EPEPS-positive samples, and 15 BT-positive samples.
Conclusion: Several exogenous genes were detected in the farmed and commercially available soybeans. We should use caution in the management of genetically modified soybeans. © 2015 Society of Chemical Industry.
Keywords: PCR detection; exogenous genes; genetically modified organisms; sequence analysis; soybean.
© 2015 Society of Chemical Industry.