In vivo observation of the pH alternation in mitochondria for various external stimuli

Lixia Cao; Zhensheng Zhao; Tao Zhang; Xudong Guo; Shuangqing Wang; Shayu Li; Yi Li; Guoqiang Yang

doi:10.1039/c5cc07118f

In vivo observation of the pH alternation in mitochondria for various external stimuli

Chem Commun (Camb). 2015 Dec 18;51(97):17324-7. doi: 10.1039/c5cc07118f.

Authors

Lixia Cao¹, Zhensheng Zhao¹, Tao Zhang¹, Xudong Guo¹, Shuangqing Wang¹, Shayu Li¹, Yi Li², Guoqiang Yang¹

Affiliations

¹ Beijing National Laboratory for Molecular Sciences, Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. gqyang@iccas.ac.cn shayuli@iccas.ac.cn.
² Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China. yili@mail.ipc.ac.cn.

PMID: 26462552
DOI: 10.1039/c5cc07118f

Abstract

The pH of mitochondria (pHm) has a close relationship with many biological processes. Here we developed a new indicator Mito-pH-1 for the ratiometric fluorescence detection of the mitochondria pH value, which has excellent tolerance to environmental change. And Mito-pH-1 has been used for the first time to monitor the change of pHm under temperature and H2O2 stimuli in living cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Humans
Hydrogen-Ion Concentration*
Mice
Mitochondria / metabolism*
Spectrometry, Fluorescence