Enhancing Pharmacokinetics, Tumor Accumulation, and Antitumor Efficacy by Elastin-Like Polypeptide Fusion of Interferon Alpha

Adv Mater. 2015 Dec 2;27(45):7320-4. doi: 10.1002/adma.201503440. Epub 2015 Oct 14.

Abstract

Genetic fusion of elastin-like polypeptide (ELP) to the C-terminus of interferon alpha (IFN) generates a well-defined IFN-ELP fusion protein with high yield and well-retained bioactivity. The fusion protein significantly enhances pharmacokinetics, tumor accumulation, and antitumor efficacy of interferon alpha in a murine cancer model.

Keywords: cancer therapy; elastin-like polypeptides; interferon; pharmacokinetics; protein delivery.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents / metabolism
  • Antineoplastic Agents / pharmacokinetics*
  • Antineoplastic Agents / therapeutic use
  • Circular Dichroism
  • Dynamic Light Scattering
  • Elastin / genetics
  • Elastin / metabolism
  • Escherichia coli / metabolism
  • Half-Life
  • Humans
  • Interferon-alpha / blood
  • Interferon-alpha / genetics
  • Interferon-alpha / metabolism*
  • Mice
  • Neoplasms / drug therapy
  • Peptides / genetics
  • Peptides / metabolism*
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / blood
  • Recombinant Fusion Proteins / pharmacokinetics*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Tissue Distribution

Substances

  • Antineoplastic Agents
  • Interferon-alpha
  • Peptides
  • Recombinant Fusion Proteins
  • Elastin