ShRNA-mediated silencing of the RFC3 gene suppress ovarian tumor cells proliferation

Int J Clin Exp Pathol. 2015 Aug 1;8(8):8968-75. eCollection 2015.


Ovarian carcinoma is one of the most common and lethal malignancies in the world. Replication factor C (RFC) plays an important role in DNA replication, DNA damage repair, and checkpoint control during cell cycle progression in all eukaryotes. Our previous study found that one unit of RFC complex, RFC3, is over-expressed in ovarian tumor tissues. However, its role in the development of ovarian carcinoma remains unclear. Western blot and real-time RT-PCR analysis were used to measure the expression of RFC3 in ovarian cancer cells. Lentivirus-mediated RFC3-specific shRNA was used to knock down RFC3 expression in ovarian cancer cells. Furthermore, the effect of RFC3 on tumor cellular proliferation and growth were examined, respectively. The expression level of RFC3 was remarkably up-regulated in ovarian cancer OVCAR-3 cells. With MTS and cell growth assays, the viability and proliferation of RFC3 knocking-down OVCAR-3 cell line were shown to be effectively restrained. Down-regulation of RFC3 expression arrested the cell cycle of OVCAR-3 cell in the S-phase and induced apoptosis. This study suggests that RFC3 may play an important role in the the process of ovarian carcinoma, and that it may be a potential biological treatment target in the future.

Keywords: Ovarian carcinoma; apoptosis; cell cycle arrest; cell growth; cell proliferation; replication factor C (RFC).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle / genetics*
  • Cell Line, Tumor
  • Cell Proliferation / genetics*
  • Female
  • Gene Silencing
  • Humans
  • Ovarian Neoplasms / genetics*
  • Ovarian Neoplasms / pathology
  • RNA, Small Interfering
  • Replication Protein C / genetics*
  • Up-Regulation


  • RFC3 protein, human
  • RNA, Small Interfering
  • Replication Protein C