Anti-inflammatory Effects of Resveratrol on Hypoxia/Reoxygenation-Induced Alveolar Epithelial Cell Dysfunction

J Agric Food Chem. 2015 Nov 4;63(43):9480-7. doi: 10.1021/acs.jafc.5b01168. Epub 2015 Oct 22.

Abstract

Reducing oxidative stress is crucial to prevent hypoxia-reoxygenation (H/R)-induced lung injury. Resveratrol has excellent antioxidant and anti-inflammatory effects, and this study investigated its role in H/R-induced type II pneumocyte dysfunction. H/R conditions increased expression of inflammatory cytokines including interleukin (IL)-1β (142.3 ± 21.2%, P < 0.05) and IL-6 (301.9 ± 35.1%, P < 0.01) in a type II alveolar epithelial cell line (A549), while the anti-inflammatory cytokine IL-10 (64.6 ± 9.8%, P < 0.05) and surfactant proteins (SPs) decreased. However, resveratrol treatment effectively inhibited these effects. H/R significantly activated an inflammatory transcription factor, nuclear factor (NF)-κB, while resveratrol significantly inhibited H/R-induced NF-κB transcription activities. To the best of our knowledge, this is the first study showing resveratrol-mediated reversal of H/R-induced inflammatory responses and dysfunction of type II pneumocyte cells in vitro. The effects of resveratrol were partially mediated by promoting SP expression and inhibiting inflammation with NF-κB pathway involvement. Therefore, our study provides new insights into mechanisms underlying the action of resveratrol in type II pneumocyte dysfunction.

Keywords: hypoxia; inflammation; reoxygenation; resveratrol; surfactant.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Inflammatory Agents / pharmacology*
  • Cell Line
  • Epithelial Cells / drug effects*
  • Epithelial Cells / immunology
  • Epithelial Cells / metabolism
  • Humans
  • Hypoxia / drug therapy*
  • Hypoxia / immunology
  • Hypoxia / metabolism
  • Interleukin-1beta / immunology
  • Interleukin-6 / immunology
  • Lung Injury / drug therapy*
  • Lung Injury / immunology
  • Lung Injury / metabolism
  • Oxygen / metabolism
  • Pulmonary Alveoli / cytology
  • Pulmonary Alveoli / drug effects*
  • Pulmonary Alveoli / immunology
  • Resveratrol
  • Stilbenes / pharmacology*

Substances

  • Anti-Inflammatory Agents
  • Interleukin-1beta
  • Interleukin-6
  • Stilbenes
  • Resveratrol
  • Oxygen