Identification of mechano-sensitive C fibre sensitization and contribution to nerve injury-induced mechanical hyperalgesia

Eur J Pain. 2016 Apr;20(4):615-25. doi: 10.1002/ejp.779. Epub 2015 Oct 2.


Background: C fibre hyperexcitability is fundamental to chronic pain development in humans and rodents; therefore, peripheral sensory neuronal sensitization plays a role in the development of mechanical hyperalgesia. However, the axonal properties and underlying mechanisms that are associated to these chronic pain states still require investigation.

Methods: Teased fibre electrophysiology of the saphenous nerve was used to identify C fibres in naïve and nerve-injured rats. C fibres were identified using electrical stimulation which further provided conduction velocity slowing profiles. From these nerve filaments evoked responses to mechanical stimuli were recorded. Vehicle or galanin were applied directly to the saphenous nerve trunk prior to stimulation.

Results: Increased levels of mechanically evoked activity in mechano-sensitive C fibres was associated to reduced conduction failure, enhanced conduction velocity latency recovery and reduced conduction velocity slowing. Mechanical hyperalgesia developed in nerve-injured animals in which mechano-sensitive C fibres demonstrated increased mechanically evoked responses and reduced rate of adaptation. Mechano-sensitive C fibres in nerve-injured animals had reduced levels of conduction velocity slowing, enhanced rate of conduction velocity recovery and reduced firing frequency failure versus naïve animals; all hallmarks of enhanced sensory neuronal excitability. Directly applying the antinociceptive agent galanin to the saphenous nerve trunk in naive animals led to increased conduction failure, reduced latency recovery rate and increased levels of conduction velocity slowing.

Discussion: Nerve injury-induced enhanced neural responses to mechanical stimulation are associated to defined parameters setout by conduction velocity slowing, mediated via axonal processing. Application of galanin inhibits axonal excitability.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Axons / physiology
  • Electric Stimulation
  • Hyperalgesia / etiology*
  • Hyperalgesia / physiopathology*
  • Male
  • Nerve Fibers, Unmyelinated / physiology*
  • Neural Conduction / physiology
  • Neurons, Afferent
  • Nociceptors / physiology
  • Peripheral Nerve Injuries / etiology*
  • Peripheral Nerve Injuries / physiopathology*
  • Rats
  • Rats, Wistar