Centella asiatica Leaf Extract Protects Against Indomethacin-Induced Gastric Mucosal Injury in Rats

J Med Food. 2016 Jan;19(1):38-46. doi: 10.1089/jmf.2015.3464. Epub 2015 Oct 15.


The present study evaluated the protective effect of Centella asiatica (gotu kola) leaf extract (CAE) against indomethacin (IND)-induced gastric mucosal injury in rats. Gastric mucosal injury was induced by the oral administration of IND to the rats after a 24 h fast. CAE (50 or 250 mg/kg) or lansoprazole (a reference drug) was orally administrated 30 min before the IND administration, and 5 h later, the stomachs were removed to quantify the lesions. Orally administered CAE significantly reduced IND-induced gastric injury. The histopathological observations (hematoxylin-eosin and Periodic acid-Schiff staining) confirmed the protection against gastric mucosal injury. Also, CAE decreased the malondialdehyde content compared to the control group. Moreover, pretreatment with CAE resulted in a significant reduction in the elevated expression of tumor necrosis factor, Cyclooxygenase (COX)-2, and inducible nitric oxide synthase. These results suggested that CAE possesses gastroprotective effects against IND-induced gastric mucosal injury, which could be attributed to its ability to inhibit lipid peroxidation and stimulate gastric mucus secretion in the rat gastric mucosa.

Keywords: Centella asiatica leaf extract; gastroprotection; indomethacin; lipid peroxidation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Centella / chemistry*
  • Cyclooxygenase 2 / genetics
  • Cyclooxygenase 2 / metabolism
  • Gastric Mucosa / drug effects*
  • Gastric Mucosa / injuries
  • Gastric Mucosa / metabolism
  • Humans
  • Indomethacin / administration & dosage*
  • Male
  • Malondialdehyde / metabolism
  • Plant Extracts / administration & dosage*
  • Plant Leaves / chemistry
  • Rats
  • Rats, Sprague-Dawley
  • Stomach Ulcer / chemically induced
  • Stomach Ulcer / drug therapy*
  • Stomach Ulcer / genetics
  • Stomach Ulcer / metabolism
  • Tumor Necrosis Factor-alpha / genetics
  • Tumor Necrosis Factor-alpha / metabolism


  • Plant Extracts
  • Tumor Necrosis Factor-alpha
  • Malondialdehyde
  • Cyclooxygenase 2
  • Indomethacin