Loop mediated isothermal amplification assay using hydroxy naphthol blue, conventional polymerase chain reaction and real-time PCR in the diagnosis of intraocular tuberculosis

P K Balne; S Basu; S Rath; M R Barik; S Sharma

doi:10.4103/0255-0857.167339

Loop mediated isothermal amplification assay using hydroxy naphthol blue, conventional polymerase chain reaction and real-time PCR in the diagnosis of intraocular tuberculosis

Indian J Med Microbiol. 2015 Oct-Dec;33(4):568-71. doi: 10.4103/0255-0857.167339.

Authors

P K Balne, S Basu, S Rath, M R Barik, S Sharma¹

Affiliation

¹ Jhaveri Microbiology Centre, L V Prasad Eye Institute, Hyderabad, Telangana, India.

PMID: 26470966
DOI: 10.4103/0255-0857.167339

Abstract

This study is a comparative evaluation (Chi-square test) of a closed tube loop mediated isothermal amplification assay using hydroxy naphthol blue dye (HNB-LAMP), real-time polymerase chain reaction (PCR) and conventional PCR in the diagnosis of intraocular tuberculosis. Considering clinical presentation as the gold standard in 33 patients, the sensitivity of HNB-LAMP assay (75.8%) was higher (not significant, P value 0.2) than conventional PCR (57.6%) and lower than real-time PCR (90.9%). Specificity was 100% by all three methods. No amplification was observed in negative controls (n = 20) by all three methods. The cost of the HNB-LAMP assay was Rs. 500.00 and it does not require thermocycler, therefore, it can be used as an alternative to conventional PCR in resource-poor settings.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Costs and Cost Analysis
Humans
Molecular Diagnostic Techniques / methods*
Nucleic Acid Amplification Techniques / methods*
Sensitivity and Specificity
Tuberculosis, Ocular / diagnosis*